RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing

Djupedal, Ingela (author): Södertörns högskola,Institutionen för livsvetenskaper,Karolinska Institutet

Bonilla, Carolina (author): Södertörns högskola,Institutionen för livsvetenskaper,Karolinska Institutet

Ekwall, Karl (author): Södertörns högskola,Institutionen för livsvetenskaper,Karolinska Institutet

(creator_code:org_t)

2005-10-03
2005
English.
In: Genes & Development. - : Cold Spring Harbor Laboratory. - 0890-9369 .- 1549-5477. ; 19:19, s. 2301-2306

Related links:: http://www.ncbi.nlm....; show more...; http://genesdev.cshl...; https://urn.kb.se/re...; https://doi.org/10.1...; http://kipublication...; show less...

Abstract Subject headings

Fission yeast centromeric repeats are transcribed into small interfering RNA (siRNA) precursors (pre-siRNAs), which are processed by Dicer to direct heterochromatin formation. Recently, Rpb1 and Rpb2 subunits of RNA polymerase II (RNA Pol II) were shown to mediate RNA interference (RNAi)-directed chromatin modification but did not affect pre-siRNA levels. Here we show that another Pol II subunit, Rpb7 has a specific role in presiRNA transcription. We define a centromeric presiRNA promoter from which initiation is exquisitely sensitive to the rpb7-G150D mutation. In contrast to other Pol II subunits, Rpb7 promotes pre-siRNA transcription required for RNAi-directed chromatin silencing.

By the author/editor: Djupedal, Ingela; Portoso, M; Spåhr, H; Bonilla, Carolin ...; Gustafsson, C M; Allshire, R C; show more...; Ekwall, Karl; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Cell Biology

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