RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing

Djupedal, IngelaSödertörns högskola,Institutionen för livsvetenskaper,Karolinska Institutet (author)

Fission yeast centromeric repeats are transcribed into small interfering RNA (siRNA) precursors (pre-siRNAs), which are processed by Dicer to direct heterochromatin formation. Recently, Rpb1 and Rpb2 subunits of RNA polymerase II (RNA Pol II) were shown to mediate RNA interference (RNAi)-directed chromatin modification but did not affect pre-siRNA levels. Here we show that another Pol II subunit, Rpb7 has a specific role in presiRNA transcription. We define a centromeric presiRNA promoter from which initiation is exquisitely sensitive to the rpb7-G150D mutation. In contrast to other Pol II subunits, Rpb7 promotes pre-siRNA transcription required for RNAi-directed chromatin silencing.

Portoso, MUniversity of Edinburgh, Edinburgh, UK (author)
Spåhr, HKarolinska Institutet (author)
Bonilla, CarolinaSödertörns högskola,Institutionen för livsvetenskaper,Karolinska Institutet(Swepub:sh)shcaba05 (author)
Gustafsson, C MKarolinska Institutet (author)
Allshire, R CUniversity of Edinburgh, Edinburgh, UK (author)
Ekwall, KarlSödertörns högskola,Institutionen för livsvetenskaper,Karolinska Institutet(Swepub:sh)SHKLEL (author)
Södertörns högskolaInstitutionen för livsvetenskaper (creator_code:org_t)

In:Genes & Development: Cold Spring Harbor Laboratory19:19, s. 2301-23060890-93691549-5477

By the author/editor: Djupedal, Ingela; Portoso, M; Spåhr, H; Bonilla, Carolin ...; Gustafsson, C M; Allshire, R C; show more...; Ekwall, Karl; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Cell Biology

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