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Structural characte...
Structural characterization of a novel branching pattern in the lipopolysaccharide from nontypeable Haemophilus influenzae
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- Månsson, Martin (author)
- Södertörns högskola,Avdelning Naturvetenskap,Karolinska Institutet
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- Hood, D W (author)
- John Radcliffe Hospital, Oxford, UK
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- Moxon, E R (author)
- John Radcliffe Hospital, Oxford, UK
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- Schweda, Elke K H (author)
- Södertörns högskola,Avdelning Naturvetenskap,Karolinska Institutet
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(creator_code:org_t)
- 2003-06-23
- 2003
- English.
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In: European Journal of Biochemistry. - : Wiley. - 0014-2956 .- 1432-1033. ; 270:14, s. 2979-2991
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Structural analysis of the lipopolysaccharide (LPS) from nontypeable Haemophilus influenzae strain 981 has been achieved using NMR spectroscopy and ESI-MS on O-deacylated LPS and core oligosaccharide (OS) material as well as by ESI-MSn on permethylated dephosphorylated OS. A heterogeneous glycoform population was identified, resulting from the variable length of the OS branches attached to the glucose residue in the common structural element of H. influenzae LPS, L-alpha-d-Hepp -(1-->2)-[P Etn-->6]-L-alpha-d-Hepp -(1-->3)-[beta-d-Glcxp-(1-->4)]-L-alpha-d-Hepp -(1-->5)-[PP Etn-->4]-alpha-Kdop -(2-->6)-Lipid A. Notably, the O-6 position of the beta-d-Glcp residue was either substituted by P Cho or the disaccharide branch beta-d-Galp-(1-->4)-d-alpha-d-Hepp, while the O-4 position was substituted by the globotetraose unit, beta-d-Galp NAc-(1-->3)-alpha-d-Galp -(1-->4)-beta-d-Galp -(1-->4)-beta-d-Glcp, or sequentially truncated versions thereof. This is the first time a branching sugar residue has been reported in the outer-core region of H. influenzae LPS. Additionally, a P Etn group was identified at O-3 of the distal heptose residue in the inner-core.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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