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Single particle refinement in electron crystallography : A pilot study
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- Koeck, Philip J. B. (author)
- Karolinska Institutet,KTH,Södertörns högskola,Institutionen för livsvetenskaper,KTH / Karoliska institutet,Strukturell bioteknik
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- Purhonen, P. (author)
- Karolinska Institutet
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- Alvang, Ronny (author)
- KTH,Skolan för teknik och hälsa (STH)
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- (creator_code:org_t)
- Elsevier BV, 2007
- 2007
- English.
- In: Journal of Structural Biology. - : Elsevier BV. - 1047-8477 .- 1095-8657. ; 160:3, s. 344-352
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Electron crystallography can be used to determine the structures of membrane proteins at near-atomic resolution in some cases. However, most electron crystallography projects remain at a resolution around 10 Å. This might be partly due to lack of flatness of many two-dimensional crystals. We have investigated this problem and suggest single particle processing of locally averaged unit cells to improve the quality and possibly the resolution of three-dimensional maps. Applying this method to the secondary transporter melibiose permease we have calculated a three-dimensional map that is clearer and easier to interpret than the map derived using purely electron-crystallographic methods.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- Crystal distortion
- Electron crystallography
- Membrane proteins
- Single particle reconstruction
- melibiose
- membrane protein
- permease
- article
- calculation
- crystal structure
- crystallography
- elementary particle
- measurement
- pilot study
- priority journal
- protein structure
- structure analysis
- three dimensional imaging
- Computer Simulation
- Crystallization
- Escherichia coli Proteins
- Fourier Analysis
- Imaging
- Three-Dimensional
- Microscopy
- Electron
- Transmission
- Models
- Molecular
- Pilot Projects
- Protein Conformation
- Symporters
- Biology
Publication and Content Type
- ref (subject category)
- art (subject category)
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- Royal Institute of Technology
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