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- Koeck, Philip J. B.Karolinska Institutet,KTH,Södertörns högskola,Institutionen för livsvetenskaper,KTH / Karoliska institutet,Strukturell bioteknik (author)
Single particle refinement in electron crystallography : A pilot study
- Article/chapterEnglish2007
Publisher, publication year, extent ...
- Elsevier BV,2007
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:sh-22889
- https://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-22889URI
- https://doi.org/10.1016/j.jsb.2007.09.001DOI
- https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-24889URI
- http://kipublications.ki.se/Default.aspx?queryparsed=id:116300405URI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- QC 20100929
- Electron crystallography can be used to determine the structures of membrane proteins at near-atomic resolution in some cases. However, most electron crystallography projects remain at a resolution around 10 Å. This might be partly due to lack of flatness of many two-dimensional crystals. We have investigated this problem and suggest single particle processing of locally averaged unit cells to improve the quality and possibly the resolution of three-dimensional maps. Applying this method to the secondary transporter melibiose permease we have calculated a three-dimensional map that is clearer and easier to interpret than the map derived using purely electron-crystallographic methods.
Subject headings and genre
- NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
- NATURVETENSKAP Biologi hsv//swe
- NATURAL SCIENCES Biological Sciences hsv//eng
- Crystal distortion
- Electron crystallography
- Membrane proteins
- Single particle reconstruction
- melibiose
- membrane protein
- permease
- article
- calculation
- crystal structure
- crystallography
- elementary particle
- measurement
- pilot study
- priority journal
- protein structure
- structure analysis
- three dimensional imaging
- Computer Simulation
- Crystallization
- Escherichia coli Proteins
- Fourier Analysis
- Imaging
- Three-Dimensional
- Microscopy
- Electron
- Transmission
- Models
- Molecular
- Pilot Projects
- Protein Conformation
- Symporters
- Biology
Added entries (persons, corporate bodies, meetings, titles ...)
- Purhonen, P.Karolinska Institutet (author)
- Alvang, RonnyKTH,Skolan för teknik och hälsa (STH)(Swepub:kth)PI000000 (author)
- Grundberg, BjörnKTH,Strukturell bioteknik(Swepub:kth)u1opp05l (author)
- Hebert, HansKarolinska Institutet,KTH,Strukturell bioteknik(Swepub:kth)u1h8ygcn (author)
- Södertörns högskolaInstitutionen för livsvetenskaper (creator_code:org_t)
Related titles
- In:Journal of Structural Biology: Elsevier BV160:3, s. 344-3521047-84771095-8657
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