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Chromatin- and temp...
Chromatin- and temperature-dependent modulation of radiation-induced double-strand breaks
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- Elmroth, Kerstin, 1970 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för särskilda specialiteter, Avdelningen för onkologi,Institute of Selected Clinical Sciences, Department of Oncology
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- Nygren, Jonas (author)
- Södertörns högskola,Avdelning Naturvetenskap,Karolinska Institute
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- Stenerlöw, B. (author)
- Uppsala universitet,Enheten för biomedicinsk strålningsvetenskap,BMS
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- Hultborn, Ragnar, 1946 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för särskilda specialiteter, Avdelningen för onkologi,Institute of Selected Clinical Sciences, Department of Oncology
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(creator_code:org_t)
- 2009-07-03
- 2003
- English.
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In: International Journal of Radiation Biology. - : Informa UK Limited. - 0955-3002 .- 1362-3095. ; 79:10, s. 809-816
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Abstract
Subject headings
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- Purpose: To investigate the influence of chromatin organization and scavenging capacity in relation to irradiation temperature on the induction of double-strand breaks (DSB) in structures derived from human diploid fibroblasts. Materials and methods: Agarose plugs with different chromatin structures (intact cells±wortmannin, permeabilized cells with condensed chromatin, nucleoids and DNA) were prepared and irradiated with X-rays at 2 or 37°C and lysed using two different lysis protocols (new ice-cold lysis or standard lysis at 37°C). Induction of DSB was determined by constant-field gel electrophoresis. Results: The dose-modifying factor (DMFtemp) for irradiation at 37 compared with 2°C was 0.92 in intact cells (i.e. more DSB induced at 2°C), but gradually increased to 1.5 in permeabilized cells, 2.2 in nucleoids and 2.6 in naked DNA, suggesting a role of chromatin organization for temperature modulation of DNA damage. In addition, DMFtemp was influenced by the presence of 0.1 M DMSO or 30 mM glutathione, but not by post-irradiation temperature. Conclusion: The protective effect of low temperature was correlated to the indirect effects of ionizing radiation and was not dependent on post-irradiation temperature. Reasons for a dose modifying factor <1 in intact cells are discussed.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- dimethyl sulfoxide
- glutathione
- naked DNA
- wortmannin
- article
- chromatin
- controlled study
- correlation analysis
- diploidy
- DNA damage
- DNA strand breakage
- fibroblast
- gel electrophoresis
- human
- human cell
- lysis
- priority journal
- radiation exposure
- regulatory mechanism
- temperature dependence
- X ray
- Androstadienes
- Cell Line
- Cryopreservation
- DNA
- Dose-Response Relationship
- Radiation
- Fibroblasts
- Humans
- Radiation Dosage
- Radiation Protection
- Relative Biological Effectiveness
- Temperature
- Androstadienes/pharmacology
- Cell Line
- Chromatin/*radiation effects
- Cryopreservation/methods
- DNA/drug effects/*radiation effects
- *DNA Damage
- *Dose-Response Relationship
- Radiation
- Fibroblasts/*radiation effects
- Humans
- Radiation Dosage
- Radiation Protection/methods
- Relative Biological Effectiveness
- *Temperature
- MEDICINE
Publication and Content Type
- ref (subject category)
- art (subject category)
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