Transient inhibition of histone deacetylase activity overcomes silencing in the mating-type region in fission yeast

(creator_code:org_t)

Springer Science and Business Media LLC, 1999
1999
English.
In: Current Genetics. - : Springer Science and Business Media LLC. - 0172-8083 .- 1432-0983. ; 35:2, s. 82-87

Related links:: https://urn.kb.se/re...; show more...; https://doi.org/10.1...; http://kipublication...; show less...

Abstract Subject headings

We have investigated the effects of inhibition of histone de-acetylase activity on silencing at the silent mating-type loci in fission yeast. Treatment of exponentially growing cells with the histone deacetylase inhibitor, trichostatin A (TSA), resulted in derepression of a marker gene inserted 150 bp distal from the silent mat3-M locus. The natural targets for the silencing mechanism in this region were only partially derepressed and the activation appeared to be asymmetric. i.e. the mat2-P cassette remained silent at concentrations that clearly partially derepressed the mat3-M cassette. We further noted that treatment of wild-type h(90) cells resulted in the generation of altered sporulation phenotypes, indicating that the treatment affected the expression of mating-type genes and/or mating-type switching. The results are discussed in the light of recent accumulated data regarding the role of deacetylation for silencing in other species.

By the author/editor: Olsson, T G S; Silverstein, Reb ...; Ekwall, Karl; Sunnerhagen, P

About the subject

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