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Transient inhibitio...
Transient inhibition of histone deacetylase activity overcomes silencing in the mating-type region in fission yeast
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- Olsson, T G S (author)
- Göteborg University
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- Silverstein, Rebecka A. (author)
- Karolinska Institutet
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- Ekwall, Karl (author)
- Karolinska Institutet
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- Sunnerhagen, P (author)
- Göteborg University
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(creator_code:org_t)
- Springer Science and Business Media LLC, 1999
- 1999
- English.
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In: Current Genetics. - : Springer Science and Business Media LLC. - 0172-8083 .- 1432-0983. ; 35:2, s. 82-87
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- We have investigated the effects of inhibition of histone de-acetylase activity on silencing at the silent mating-type loci in fission yeast. Treatment of exponentially growing cells with the histone deacetylase inhibitor, trichostatin A (TSA), resulted in derepression of a marker gene inserted 150 bp distal from the silent mat3-M locus. The natural targets for the silencing mechanism in this region were only partially derepressed and the activation appeared to be asymmetric. i.e. the mat2-P cassette remained silent at concentrations that clearly partially derepressed the mat3-M cassette. We further noted that treatment of wild-type h(90) cells resulted in the generation of altered sporulation phenotypes, indicating that the treatment affected the expression of mating-type genes and/or mating-type switching. The results are discussed in the light of recent accumulated data regarding the role of deacetylation for silencing in other species.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- trichostatin A
- repression
- Schizosaccharomyces pombe
- heterochromatin
Publication and Content Type
- ref (subject category)
- art (subject category)
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