onr:"swepub:oai:DiVA.org:sh-5616"
Search: onr:"swepub:oai:DiVA.org:sh-5616" > Screening of Mhc va...
- 1 of 1
- Previous record
- Next record
- To hitlist
Screening of Mhc variation in Atlantic salmon (Salmo salar) : a comparison of restriction fragment length polymorphism (RFLP), denaturing gradient gel electrophoresis (DGGE) and sequencing
-
- Langefors, Åsa (author)
- Lund University,Lunds universitet,Biodiversitet,Biologiska institutionen,Naturvetenskapliga fakulteten,Biodiversity,Department of Biology,Faculty of Science
-
- Lohm, Jakob (author)
- Lund University,Lunds universitet,Biologiska institutionen,Naturvetenskapliga fakulteten,Department of Biology,Faculty of Science
-
- von Schantz, Torbjörn (author)
- Lund University,Lunds universitet,MEMEG,Biologiska institutionen,Naturvetenskapliga fakulteten,Department of Biology,Faculty of Science
- show more...
- show less...
- (creator_code:org_t)
- Wiley, 2000
- 2000
- English.
- In: Molecular Ecology. - : Wiley. - 0962-1083 .- 1365-294X. ; 9:2, s. 215-219
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- We compared three different molecular methods currently used for screening of Mhc variation in population studies of Atlantic salmon. Restriction fragment length polymorphism (RFLP) of the entire class II gene detected 22 haplotypes. Seventeen exon 2 sequences were obtained from individuals carrying the 22 haplotypes, two of which had not been detected by RFLP. The six alleles (27%) detected by RFLP and not by exon 2 sequencing probably resulted from sequence variation outside exon 2. Within exon 2, RFLP differentiated 88% of the sequences. Alternatively, denaturing gradient gel electrophoresis (DGGE) performed under two run conditions detected 94% of the sequence variation. Both RFLP using different probes, and the two PCR-based methods using three different primer pairs, suggest that there is only a single Mhc class II B gene in the Baltic populations of Atlantic salmon.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Ekologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Ecology (hsv//eng)
Keyword
- Biology
- Biologi
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Molecular Ecology (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Langefors, Åsa
- Lohm, Jakob
- von Schantz, Tor ...
- Grahn, Mats
- About the subject
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Biological Scien ...
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Biological Scien ...
- and Ecology
- Articles in the publication
- Molecular Ecolog ...
- By the university
- Södertörn University
- Lund University
- Karolinska Institutet
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
