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Glutathione transfe...
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Kjellander, MarcusUppsala universitet,Biokemi
(author)
Glutathione transferases immobilized on nanoporous alumina : Flow system kinetics, screening, and stability
- Article/chapterEnglish2014
Publisher, publication year, extent ...
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Elsevier BV,2014
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:su-100853
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-100853URI
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https://doi.org/10.1016/j.ab.2013.10.004DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-210099URI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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AuthorCount:5;
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The previously uncharacterized Drosophila melanogaster Epsilon-class glutathione transferases E6 and E7 were immobilized on nanoporous alumina. The nanoporous anodized alumina membranes were derivatized with 3-aminopropyl-triethoxysilane, and the amino groups were activated with carbonyldiimidazole to allow coupling of the enzymes via c-amino groups. Kinetic analyses of the immobilized enzymes were carried out in a circulating flow system using CDNB (1-chloro-2,4-dinitrobenzene) as substrate, followed by specificity screening with alternative substrates. A good correlation was observed between the substrate screening data for immobilized enzyme and corresponding data for the enzyme in solution. A limited kinetic study was also carried out on immobilized human GST S1-1 (also known as hematopoietic prostaglandin D synthase). The stability of the immobilized enzymes was virtually identical to that of enzymes in solution, and no leakage of enzyme from the matrix could be observed.
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Mazari, Aslam M. A.Stockholms universitet,Institutionen för neurokemi,Department of Neurochemistry, Stockholm University, SE-10691, Sweden(Swepub:su)muas4460
(author)
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Boman, MatsUppsala universitet,Oorganisk kemi(Swepub:uu)matsboma
(author)
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Mannervik, BengtUppsala universitet,Stockholms universitet,Institutionen för neurokemi,Uppsala University, Sweden,Biokemi(Swepub:uu)bengmann
(author)
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Johansson, GunnarUppsala universitet,Biokemi(Swepub:uu)gunnjoha
(author)
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Uppsala universitetBiokemi
(creator_code:org_t)
Related titles
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In:Analytical Biochemistry: Elsevier BV446, s. 59-630003-26971096-0309
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