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Suppressing one-bon...
Suppressing one-bond homonuclear 13C,13C scalar couplings in the J-HMBC NMR experiment : application to 13C site-specifically labeled oligosaccharides
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- Pendrill, Robert (author)
- Stockholms universitet,Institutionen för organisk kemi
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- Sørensen, Ole W. (author)
- Stockholms universitet,Institutionen för organisk kemi
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- Widmalm, Göran (author)
- Stockholms universitet,Institutionen för organisk kemi
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(creator_code:org_t)
- 2014-01-07
- 2014
- English.
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In: Magnetic Resonance in Chemistry. - : Wiley. - 0749-1581 .- 1097-458X. ; 52:3, s. 82-86
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Abstract
Subject headings
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- Site-specific C-13 isotope labeling is a useful approach that allows for the measurement of homonuclear C-13,C-13 coupling constants. For three site-specifically labeled oligosaccharides, it is demonstrated that using the J-HMBC experiment for measuring heteronuclear long-range coupling constants is problematical for the carbons adjacent to the spin label. By incorporating either a selective inversion pulse or a constant-time element in the pulse sequence, the interference from one-bond C-13,C-13 scalar couplings is suppressed, allowing the coupling constants of interest to be measured without complications. Experimental spectra are compared with spectra of a nonlabeled compound as well as with simulated spectra. The work extends the use of the J-HMBC experiments to site-specifically labeled molecules, thereby increasing the number of coupling constants that can be obtained from a single preparation of a molecule.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
Keyword
- NMR
- 1H
- 13C
- J-HMBC
- oligosaccharide
- heteronuclear long-range coupling constants
- site-specific labeling
Publication and Content Type
- ref (subject category)
- art (subject category)
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