Search: onr:"swepub:oai:DiVA.org:su-124913" > Peptide nanoparticl...
Fältnamn | Indikatorer | Metadata |
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000 | 04270naa a2200469 4500 | |
001 | oai:DiVA.org:su-124913 | |
003 | SwePub | |
008 | 160106s2015 | |||||||||||000 ||eng| | |
009 | oai:prod.swepub.kib.ki.se:130935549 | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-1249132 URI |
024 | 7 | a https://doi.org/10.1089/nat.2014.05112 DOI |
024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1309355492 URI |
040 | a (SwePub)sud (SwePub)ki | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Järver, Peteru Medical Research Council , Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, United Kingdom4 aut |
245 | 1 0 | a Peptide nanoparticle delivery of charge-neutral splice-switching morpholino oligonucleotides. |
264 | 1 | b Mary Ann Liebert Inc,c 2015 |
338 | a print2 rdacarrier | |
520 | a Oligonucleotide analogs have provided novel therapeutics targeting various disorders. However, their poor cellular uptake remains a major obstacle for their clinical development. Negatively charged oligonucleotides, such as 2'-O-Methyl RNA and locked nucleic acids have in recent years been delivered successfully into cells through complex formation with cationic polymers, peptides, liposomes, or similar nanoparticle delivery systems. However, due to the lack of electrostatic interactions, this promising delivery method has been unsuccessful to date using charge-neutral oligonucleotide analogs. We show here that lipid-functionalized cell-penetrating peptides can be efficiently exploited for cellular transfection of the charge-neutral oligonucleotide analog phosphorodiamidate morpholino. The lipopeptides form complexes with splice-switching phosphorodiamidate morpholino oligonucleotide and can be delivered into clinically relevant cell lines that are otherwise difficult to transfect while retaining biological activity. To our knowledge, this is the first study to show delivery through complex formation of biologically active charge-neutral oligonucleotides by cationic peptides. | |
650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
700 | 1 | a Zaghloul, Eman Mu Karolinska Institutet4 aut |
700 | 1 | a Arzumanov, Andrey Au Medical Research Council, Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, United Kingdom4 aut |
700 | 1 | a Saleh, Amer Fu Medical Research Council, Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, United Kingdom4 aut |
700 | 1 | a McClorey, Grahamu Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom4 aut |
700 | 1 | a Hammond, Suzan Mu Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom4 aut |
700 | 1 | a Hällbrink, Mattiasu Department of Laboratory Medicine, Karolinska Institute, Karolinska University Hospital, Huddinge, Sweden4 aut0 (Swepub:su)mhall |
700 | 1 | a Langel, Ülou Stockholms universitet,Institutionen för neurokemi4 aut0 (Swepub:su)ulo |
700 | 1 | a Smith, C I Edvardu Karolinska Institutet4 aut |
700 | 1 | a Wood, Matthew J Au Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United Kingdom4 aut |
700 | 1 | a Gait, Michael Ju Medical Research Council, Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, United Kingdom4 aut |
700 | 1 | a El Andaloussi, Samiru Karolinska Institutet4 aut |
710 | 2 | a Karolinska Institutetb Medical Research Council , Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, United Kingdom4 org |
773 | 0 | t Nucleic Acid Therapeuticsd : Mary Ann Liebert Incg 25:2, s. 65-77q 25:2<65-77x 2159-3337x 2159-3345 |
856 | 4 | u https://doi.org/10.1089/nat.2014.0511y Fulltext |
856 | 4 | u https://www.liebertpub.com/doi/pdf/10.1089/nat.2014.0511 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-124913 |
856 | 4 8 | u https://doi.org/10.1089/nat.2014.0511 |
856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:130935549 |
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