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Targeted DNA sequencing and in situ mutation analysis using mobile phone microscopy
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- Kühnemund, Malte (author)
- Uppsala universitet,Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Uppsala University, Sweden,Molekylära verktyg,Science for Life Laboratory, SciLifeLab,Stockholm Univ, Dept Biophys & Biochem, Sci Life Lab, Box 1031, SE-17121 Solna, Sweden.
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- Wei, Qingshan (author)
- Univ Calif Los Angeles, Dept Elect Engn, Los Angeles, CA 90095 USA.;Univ Calif Los Angeles, Dept Bioengn, Los Angeles, CA 90095 USA.
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- Darai, Evangelia (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Stockholm Univ, Dept Biophys & Biochem, Sci Life Lab, Box 1031, SE-17121 Solna, Sweden.
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- (creator_code:org_t)
- 2017-01-17
- 2017
- English.
- In: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 8
- Journal article (peer-reviewed)
Abstract
Subject headings
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- Molecular diagnostics is typically outsourced to well-equipped centralized laboratories, often far from the patient. We developed molecular assays and portable optical imaging designs that permit on-site diagnostics with a cost-effective mobile-phone-based multimodal microscope. We demonstrate that targeted next-generation DNA sequencing reactions and in situ point mutation detection assays in preserved tumour samples can be imaged and analysed using mobile phone microscopy, achieving a new milestone for tele-medicine technologies.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
Keyword
- Biochemistry
- biokemi
Publication and Content Type
- ref (subject category)
- art (subject category)
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- Kühnemund, Malte
- Wei, Qingshan
- Darai, Evangelia
- Wang, Yingjie
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- Yang, Zhao
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- Tseng, Derek
- Ahlford, Annika
- Mathot, Lucy
- Sjöblom, Tobias
- Ozcan, Aydogan
- Nilsson, Mats
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