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Enhanced susceptibi...
Enhanced susceptibility of obese mice to glycidamide-induced sperm chromatin damage without increased oxidative stress
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Gutzkow, K. B. (author)
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Duale, N. (author)
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Danielsen, T. (author)
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- von Stedingk, Hans (author)
- Stockholms universitet,Institutionen för miljövetenskap och analytisk kemi
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Shahzadi, S. (author)
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Instanes, C. (author)
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Olsen, A. -K. (author)
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Steffensen, I. -L. (author)
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Hofer, T. (author)
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- Törnqvist, Margareta (author)
- Stockholms universitet,Institutionen för miljövetenskap och analytisk kemi
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Brunborg, G. (author)
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Lindeman, B. (author)
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(creator_code:org_t)
- 2016-08-30
- 2016
- English.
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In: Andrology. - : Wiley. - 2047-2919 .- 2047-2927. ; 4:6, s. 1092-1114
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Diet-induced obesity is known to impair male reproduction and may aggravate the male reproductive toxicity of the food contaminant acrylamide. Exposure of male mice to acrylamide induces paternally mediated pre- and post-implantation losses because of spermatozoal toxicity and these effects are potentiated in mice fed a high-fat diet. Glycidamide - an acrylamide metabolite - is the primary mediator of reproductive effects in males. The mechanisms causing the interaction between diet and acrylamide are not clear. However, diet-induced obesity is associated with oxidative stress in male reproductive tissues which might contribute to increased germ cell susceptibility. In this study, we investigated whether a moderate diet-induced obesity regimen could interfere with glycidamide-induced spermatozoal toxicity and increase oxidative stress. For this purpose, sperm chromatin integrity, oxidised DNA and protein levels, transcript levels of oxidative stress responsive genes and glycidamide-induced DNA and haemoglobin adducts were analysed in samples from male mice exposed to a high-fat diet for 6 weeks in combination with a single glycidamide exposure 7 days prior to sacrifice. We found that glycidamide-induced sperm DNA fragmentation was markedly higher in obese than in lean mice. However, the levels of oxidised DNA and/or protein in blood, liver and testicular tissue was lower in obese than in lean mice. Accompanying the reduced level of oxidised macromolecules, the transcript levels of several oxidative stress-related genes were altered in the liver and testis from obese mice suggesting induction of an antioxidant response in these animals. The haemoglobin-glycidamide adduct levels were higher in obese than in lean animals, whereas obesity did not seem to increase the level of glycidamide-induced DNA adducts. These findings show that a moderate diet-induced obesity regimen may potentiate glycidamide-induced sperm cells toxicity and suggest that the increase in glycidamide-induced sperm toxicity observed in obese mice does not depend on overt oxidative stress.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reproduktionsmedicin och gynekologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Obstetrics, Gynaecology and Reproductive Medicine (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- DNA damage
- gene expression
- glycidamide
- obesity
- oxidative stress
- sperm chromatin integrity
Publication and Content Type
- ref (subject category)
- art (subject category)
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Andrology
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- By the author/editor
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Gutzkow, K. B.
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Duale, N.
-
Danielsen, T.
-
von Stedingk, Ha ...
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Shahzadi, S.
-
Instanes, C.
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show more...
-
Olsen, A. -K.
-
Steffensen, I. - ...
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Hofer, T.
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Törnqvist, Marga ...
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Brunborg, G.
-
Lindeman, B.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Obstetrics Gynae ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
- Articles in the publication
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Andrology
- By the university
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Stockholm University