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Generation of a mul...
Generation of a multipurpose Prdm16 mouse allele by targeted gene trapping
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Strassman, Alexander (author)
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Schnuetgen, Frank (author)
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- Dai, Qi (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
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Jones, Jennifer C. (author)
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Gomez, Angela C. (author)
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Pitstick, Lenore (author)
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Holton, Nathan E. (author)
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Moskal, Russell (author)
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Leslie, Erin R. (author)
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von Melchner, Harald (author)
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Beier, David R. (author)
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Bjork, Bryan C. (author)
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(creator_code:org_t)
- 2017-01-01
- 2017
- English.
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In: Disease Models and Mechanisms. - : The Company of Biologists. - 1754-8403 .- 1754-8411. ; 10:7, s. 909-922
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https://doi.org/10.1...
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http://dmm.biologist...
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Abstract
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- Gene trap mutagenesis is a powerful tool to create loss-of-function mutations in mice and other model organisms. Modifications of traditional gene trap cassettes, including addition of conditional features in the form of Flip-excision (FlEx) arrays to enable directional gene trap cassette inversions by Cre and Flpe site-specific recombinases, greatly enhanced their experimental potential. By taking advantage of these conditional gene trap cassettes, we developed a generic strategy for generating conditional mutations and validated this strategy in mice carrying a multipurpose allele of the Prdm16 transcription factor gene. We demonstrate that the gene trap insertion creates a null mutation replicating the Pierre Robin sequence-type cleft palate phenotype of other Prdm16 mutant mice. Consecutive breeding to Flpe and Emx1(IREScre) deleter mice spatially restricted Prdm16 loss to regions of the forebrain expressing the homeobox gene Emx1, demonstrating the utility of the technology for the analysis of tissue-specific gene functions.
Subject headings
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Keyword
- Conditional gene trap
- Cleft palate
- Mandible
- Micrognathia
- Pierre Robin sequence
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Strassman, Alexa ...
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Schnuetgen, Fran ...
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Dai, Qi
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Jones, Jennifer ...
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Gomez, Angela C.
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Pitstick, Lenore
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show more...
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Holton, Nathan E ...
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Moskal, Russell
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Leslie, Erin R.
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von Melchner, Ha ...
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Beier, David R.
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Bjork, Bryan C.
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Cell Biology
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Medical Biotechn ...
- Articles in the publication
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Disease Models a ...
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Stockholm University