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Role of autophagy i...
Role of autophagy in cell-penetrating peptide transfection model
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- Dowaidar, Moataz (author)
- Stockholms universitet,Institutionen för neurokemi
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- Gestin, Maxime (author)
- Stockholms universitet,Institutionen för neurokemi
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- Cerrato, Carmine Pasquale (author)
- Stockholms universitet,Institutionen för neurokemi
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- Jafferali, Mohammed Hakim (author)
- Stockholms universitet,Institutionen för neurokemi
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Margus, Helerin (author)
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Kivistik, Paula Ann (author)
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- Ezzat, Kariem (author)
- Karolinska Institutet
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- Hallberg, Einar (author)
- Stockholms universitet,Institutionen för neurokemi
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Pooga, Margus (author)
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- Hällbrink, Mattias (author)
- Stockholms universitet,Institutionen för neurokemi
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- Langel, Ülo (author)
- Stockholms universitet,Institutionen för neurokemi,University of Tartu, Estonia
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(creator_code:org_t)
- 2017-10-03
- 2017
- English.
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In: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 7
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https://doi.org/10.1...
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https://www.nature.c...
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Abstract
Subject headings
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- Cell-penetrating peptides (CPPs) uptake mechanism is still in need of more clarification to have a better understanding of their action in the mediation of oligonucleotide transfection. In this study, the effect on early events (1 h treatment) in transfection by PepFect14 (PF14), with or without oligonucleotide cargo on gene expression, in HeLa cells, have been investigated. The RNA expression profile was characterized by RNA sequencing and confirmed by qPCR analysis. The gene regulations were then related to the biological processes by the study of signaling pathways that showed the induction of autophagy-related genes in early transfection. A ligand library interfering with the detected intracellular pathways showed concentration-dependent effects on the transfection efficiency of splice correction oligonucleotide complexed with PepFect14, proving that the autophagy process is induced upon the uptake of complexes. Finally, the autophagy induction and colocalization with autophagosomes have been confirmed by confocal microscopy and transmission electron microscopy. We conclude that autophagy, an inherent cellular response process, is triggered by the cellular uptake of CPP-based transfection system. This finding opens novel possibilities to use autophagy modifiers in future gene therapy.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- neurokemi med molekylär neurobiologi
- Neurochemistry with Molecular Neurobiology
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Dowaidar, Moataz
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Gestin, Maxime
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Cerrato, Carmine ...
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Jafferali, Moham ...
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Margus, Helerin
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Kivistik, Paula ...
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show more...
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Ezzat, Kariem
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Hallberg, Einar
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Pooga, Margus
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Hällbrink, Matti ...
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Langel, Ülo
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
- Articles in the publication
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Scientific Repor ...
- By the university
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Stockholm University
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Karolinska Institutet