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c-Jun supports ribo...
c-Jun supports ribosomal RNA processing and nucleolar localization of RNA helicase DDX21
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Holmström, Tim H. (author)
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Mialon, Antoine (author)
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Kallio, Marko (author)
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Nymalm, Yvonne (author)
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Mannermaa, Leni (author)
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- Holm, Tina (author)
- Stockholms universitet,Institutionen för neurokemi
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- Johansson, Henrik (author)
- Stockholms universitet,Institutionen för neurokemi
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Black, Elizabeth (author)
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Gillespie, David (author)
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Salminen, Tiina A. (author)
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- Langel, Ülo (author)
- Stockholms universitet,Institutionen för neurokemi
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Valdez, Benigno C. (author)
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Westermarck, Jukka (author)
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(creator_code:org_t)
- 2008
- 2008
- English.
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In: Journal of Biological Chemistry. - 0021-9258 .- 1083-351X. ; 283:11, s. 7046-7053
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The molecular mechanisms by which the AP-1 transcription factor c-Jun exerts its biological functions are not clearly understood. In addition to its well established role in transcriptional regulation of gene expression, several reports have suggested that c-Jun may also regulate cell behavior by non-transcriptional mechanisms. Here, we report that small interfering RNA-mediated depletion of c-Jun from mammalian cells results in inhibition of 28 S and 18 S rRNA accumulation. Moreover, we show that c-Jun depletion results in partial translocation of RNA helicase DDX21, implicated in rRNA processing, from the nucleolus to the nucleoplasm. We demonstrate that DDX21 translocation is rescued by exogenous c-Jun expression and that c-Jun depletion inhibits rRNA binding of DDX21. Furthermore, the direct interaction between c-Jun and DDX21 regulates nucleolar localization of DDX21. These results demonstrate that in addition to its transcriptional effects, c-Jun regulates rRNA processing and nucleolar compartmentalization of the rRNA processing protein DDX21. Thus, our results demonstrate a nucleolar mechanism through which c-Jun can regulate cell behavior. Moreover, these results suggest that the phenotypes observed previously in c-Jun-depleted mouse models and cell lines could be partly due to the effects of c-Jun on rRNA processing.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Holmström, Tim H ...
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Mialon, Antoine
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Kallio, Marko
-
Nymalm, Yvonne
-
Mannermaa, Leni
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Holm, Tina
-
show more...
-
Johansson, Henri ...
-
Black, Elizabeth
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Gillespie, David
-
Salminen, Tiina ...
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Langel, Ülo
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Valdez, Benigno ...
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Westermarck, Juk ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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Journal of Biolo ...
- By the university
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Stockholm University