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Mitotic spindle ass...
Mitotic spindle assembly and γ-tubulin localisation depend on the integral nuclear membrane protein, Samp1
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- Larsson, Veronica J. (författare)
- Stockholms universitet,Institutionen för neurokemi
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- Jafferali, Mohammed Hakim (författare)
- Stockholms universitet,Institutionen för neurokemi
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- Vijayaraghavan, Balaje (författare)
- Stockholms universitet,Institutionen för neurokemi
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- Figueroa, Ricardo A. (författare)
- Stockholms universitet,Institutionen för neurokemi
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- Hallberg, Einar (författare)
- Stockholms universitet,Institutionen för neurokemi
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(creator_code:org_t)
- 2018-01-01
- 2018
- Engelska.
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Ingår i: Journal of Cell Science. - : The Company of Biologists. - 0021-9533 .- 1477-9137. ; 131:8
- Relaterad länk:
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https://doi.org/10.1...
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https://jcs.biologis...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We have investigated a possible role of the inner nuclear membrane protein, Samp1, in the mitotic machinery. Live cell imaging showed that Samp1aYFP distributed as filamentous structures in the mitotic spindle, partially co-localising with ß-tubulin. Samp1 depletion resulted in an increased frequency of cells with signs of chromosomal mis-segregation and prolonged metaphase, indicating problems with spindle assembly and/or chromosomal alignment. Consistently, mitotic spindles in Samp1 depleted cells contained significantly lower levels of ß-tubulin and γ-tubulin, phenotypes which were rescued by overexpression of Samp1aYFP. We found that Samp1 can bind directly to γ-tubulin and that Samp1 co-precipitated with γ-tubulin and HAUS6 of the Augmin complex in live cells. The levels of Haus6, in the mitotic spindle also decreased after Samp1 depletion. We show that Samp1 is involved in the recruitment of Haus6 and γ-tubulin to the mitotic spindle. Samp1 is the first inner nuclear membrane protein shown to have a function in mitotic spindle assembly.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Nyckelord
- Samp1
- nuclear membrane
- mitotic spindle
- gamma tubulin
- Augmin
- Cancer
- neurokemi med molekylär neurobiologi
- Neurochemistry with Molecular Neurobiology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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