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A novel system to m...
A novel system to monitor mitochondrial translation in yeast
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- Suhm, Tamara (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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Habernig, Lukas (author)
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- Rzepka, Magdalena (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Kaimal, Jayasankar Mohanakrishnan (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
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- Andréasson, Claes (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
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- Büttner, Sabrina (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,University of Graz, Austria
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- Ott, Martin (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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(creator_code:org_t)
- 2018-03-05
- 2018
- English.
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In: Microbial Cell. - : Shared Science Publishers OG. - 2311-2638. ; 5:3, s. 158-164
- Related links:
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https://doi.org/10.1...
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http://microbialcell...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
Close
- The mitochondrial genome is responsible for the production of a handful of polypeptides that are core subunits of the membrane-bound oxidative phosphorylation system. Until now the mechanistic studies of mitochondrial protein synthesis inside cells have been conducted with inhibition of cytoplasmic protein synthesis to reduce the background of nuclear gene expression with the undesired consequence of major disturbances of cellular signaling cascades. Here we have generated a system that allows direct monitoring of mitochondrial translation in unperturbed cells. A recoded gene for superfolder GFP was inserted into the yeast (Saccharomyces cerevisiae) mitochondrial genome and enabled the detection of translation through fluorescence microscopy and flow cytometry in functional mitochondria. This novel tool allows the investigation of the function and regulation of mitochondrial translation during stress signaling, aging and mitochondrial biogenesis.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- mitochondrial translation
- flow cytometry
- superfolder GFP
- strain engineering
- biokemi
- Biochemistry
Publication and Content Type
- ref (subject category)
- art (subject category)
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