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- Dowaidar, MoatazStockholms universitet,Institutionen för biokemi och biofysik (author)
Chitosan enhances gene delivery of oligonucleotide complexes with magnetic nanoparticles–cell-penetrating peptide
- Article/chapterEnglish2018
Publisher, publication year, extent ...
- 2018-09-17
- SAGE Publications,2018
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:su-160327
- https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-160327URI
- https://doi.org/10.1177/0885328218796623DOI
Supplementary language notes
- Language:English
- Summary in:English
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- SwePubSwePub
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Notes
- Gene-based therapies, including the delivery of oligonucleotides, offer promising methods for the treatment of cancer cells. However, they have various limitations including low efficiency. Herein, cell-penetrating peptides (CPPs)-conjugated chitosan-modified iron oxide magnetic nanoparticles (CPPs-CTS@MNPs) with high biocompatibility as well as high efficiency were tested for the delivery of oligonucleotides such as plasmid pGL3, splice correction oligonucleotides, and small-interfering RNA. A biocompatible nanocomposite, in which CTS@MNPs was incorporated in non-covalent complex with CPPs-oligonucleotide, is developed. Modifying the surface of magnetic nanoparticles with cationic chitosan-modified iron oxide improved the performance of magnetic nanoparticles-CPPs for oligonucleotide delivery. CPPs-CTS@MNPs complexes enhance oligonucleotide transfection compared to CPPs@MNPs or CPPs. The hydrophilic character of CTS@MNPs improves complexation with plasmid pGL3, splice correction oligonucleotides, and small-interfering RNA payload, which consequently resulted in not only strengthening the colloidal stability of the constructed complex but also improving their biocompatibility. Transfection using PF14-splice correction oligonucleotides-CTS@MNPs showed sixfold increase of the transfection compared to splice correction oligonucleotides-PF14 that showed higher transfection than the commercially available lipid-based vector Lipofectamine™ 2000. Nanoscaled CPPs-CTS@MNPs comprise a new family of biomaterials that can circumvent some of the limitations of CPPs or magnetic nanoparticles.
Subject headings and genre
- NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
- Cell-penetrating peptides
- magnetic nanoparticles
- chitosan
- gene delivery
- small-interfering RNA
- splice correction oligonucleotides
Added entries (persons, corporate bodies, meetings, titles ...)
- Abdelhamid, Hani NasserStockholms universitet,Institutionen för material- och miljökemi (MMK),Assuit University, Egypt(Swepub:su)hnass (author)
- Hällbrink, MattiasStockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)mhall (author)
- Langel, ÜloStockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)ulo (author)
- Zou, XiaodongStockholms universitet,Institutionen för material- och miljökemi (MMK)(Swepub:su)xzou (author)
- Stockholms universitetInstitutionen för biokemi och biofysik (creator_code:org_t)
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- In:Journal of biomaterials applications: SAGE Publications33:3, s. 392-4010885-32821530-8022
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