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NanoJ :
NanoJ : a high-performance open-source super-resolution microscopy toolbox
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Laine, Romain F. (author)
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Tosheva, Kalina L. (author)
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Gustafsson, Nils (author)
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Gray, Robert D. M. (author)
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Almada, Pedro (author)
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Albrecht, David (author)
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Risa, Gabriel T. (author)
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- Hurtig, Fredrik (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
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- Lindås, Ann-Christin (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
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Baum, Buzz (author)
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Mercer, Jason (author)
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Leterrier, Christophe (author)
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Pereira, Pedro M. (author)
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Culley, Sian (author)
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Henriques, Ricardo (author)
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(creator_code:org_t)
- 2019-02-18
- 2019
- English.
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In: Journal of Physics D. - : IOP Publishing. - 0022-3727 .- 1361-6463. ; 52:16
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Abstract
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- Super-resolution microscopy (SRM) has become essential for the study of nanoscale biological processes. This type of imaging often requires the use of specialised image analysis tools to process a large volume of recorded data and extract quantitative information. In recent years, our team has built an open-source image analysis framework for SRM designed to combine high performance and ease of use. We named it NanoJ-a reference to the popular ImageJ software it was developed for. In this paper, we highlight the current capabilities of NanoJ for several essential processing steps: spatio-temporal alignment of raw data (NanoJ-Core), super-resolution image reconstruction (NanoJ-SRRF), image quality assessment (NanoJ-SQUIRREL), structural modelling (NanoJ-VirusMapper) and control of the sample environment (NanoJ-Fluidics). We expect to expand NanoJ in the future through the development of new tools designed to improve quantitative data analysis and measure the reliability of fluorescent microscopy studies.
Subject headings
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
Keyword
- super-resolution microscopy
- ImageJ
- Fiji
- image analysis
- image quality assessment
- fluidics
- single-particle analysis
Publication and Content Type
- ref (subject category)
- for (subject category)
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- By the author/editor
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Laine, Romain F.
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Tosheva, Kalina ...
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Gustafsson, Nils
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Gray, Robert D. ...
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Almada, Pedro
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Albrecht, David
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show more...
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Risa, Gabriel T.
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Hurtig, Fredrik
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Lindås, Ann-Chri ...
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Baum, Buzz
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Mercer, Jason
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Leterrier, Chris ...
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Pereira, Pedro M ...
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Culley, Sian
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Henriques, Ricar ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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Journal of Physi ...
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Stockholm University