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  • Bergqvist, CeciliaStockholms universitet,Institutionen för biokemi och biofysik (author)

Monitoring of chromatin organization in live cells by FRIC. Effects of the inner nuclear membrane protein Samp1

  • Article/chapterEnglish2019

Publisher, publication year, extent ...

  • 2019-02-22
  • Oxford University Press (OUP),2019
  • electronicrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:su-168660
  • https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-168660URI
  • https://doi.org/10.1093/nar/gkz123DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:141265123URI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • In most cells, transcriptionally inactive heterochromatin is preferentially localized in the nuclear periphery and transcriptionally active euchromatin is localized in the nuclear interior. Different cell types display characteristic chromatin distribution patterns, which change dramatically during cell differentiation, proliferation, senescence and different pathological conditions. Chromatin organization has been extensively studied on a cell population level, but there is a need to understand dynamic reorganization of chromatin at the single cell level, especially in live cells. We have developed a novel image analysis tool that we term Fluorescence Ratiometric Imaging of Chromatin (FRIC) to quantitatively monitor dynamic spatiotemporal distribution of euchromatin and total chromatin in live cells. A vector (pTandemH) assures stoichiometrically constant expression of the histone variants Histone 3.3 and Histone 2B, fused to EGFP and mCherry, respectively. Quantitative ratiometric (H3.3/H2B) imaging displayed a concentrated distribution of heterochromatin in the periphery of U2OS cell nuclei. As proof of concept, peripheral heterochromatin responded to experimental manipulation of histone acetylation. We also found that peripheral heterochromatin depended on the levels of the inner nuclear membrane protein Samp1, suggesting an important role in promoting peripheral heterochromatin. Taken together, FRIC is a powerful and robust new tool to study dynamic chromatin redistribution in live cells.

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Added entries (persons, corporate bodies, meetings, titles ...)

  • Niss, FridaStockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)fniss (author)
  • Figueroa, Ricardo A.Stockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)rfigu (author)
  • Beckman, MarieStockholms universitet,Institutionen för biokemi och biofysik,Karolinska Institutet, Sweden (author)
  • Maksel, Danuta (author)
  • Jafferali, Mohammed H.Stockholms universitet,Institutionen för biokemi och biofysik (author)
  • Kulyté, AgnéKarolinska Institutet (author)
  • Ström, Anna-LenaStockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)astr (author)
  • Hallberg, EinarStockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)eihal (author)
  • Stockholms universitetInstitutionen för biokemi och biofysik (creator_code:org_t)

Related titles

  • In:Nucleic Acids Research: Oxford University Press (OUP)47:90305-10481362-4962

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