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Discovery of glycer...
Discovery of glycerol phosphate modification on streptococcal rhamnose polysaccharides
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Edgar, Rebecca J. (author)
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van Hensbergen, Vincent P. (author)
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- Ruda, Alessandro (author)
- Stockholms universitet,Institutionen för organisk kemi
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Turner, Andrew G. (author)
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Deng, Pan (author)
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Le Breton, Yoann (author)
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El-Sayed, Najib M. (author)
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Belew, Ashton T. (author)
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McIver, Kevin S. (author)
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McEwan, Alastair G. (author)
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Morris, Andrew J. (author)
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Lambeau, Gérard (author)
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Walker, Markj (author)
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Rush, Jeffrey S. (author)
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Korotkov, Konstantin (author)
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- Widmalm, Göran (author)
- Stockholms universitet,Institutionen för organisk kemi
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van Sorge, Nina M. (author)
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Korotkova, Natalia (author)
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(creator_code:org_t)
- 2019-04-01
- 2019
- English.
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In: Nature Chemical Biology. - : Springer Science and Business Media LLC. - 1552-4450 .- 1552-4469. ; 15:5, s. 463-
- Related links:
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Cell wall glycopolymers on the surface of Gram-positive bacteria are fundamental to bacterial physiology and infection biology. Here we identify gacH, a gene in the Streptococcus pyogenes group A carbohydrate (GAC) biosynthetic cluster, in two independent transposon library screens for its ability to confer resistance to zinc and susceptibility to the bactericidal enzyme human group IIA-secreted phospholipase A(2). Subsequent structural and phylogenetic analysis of the GacH extracellular domain revealed that GacH represents an alternative class of glycerol phosphate transferase. We detected the presence of glycerol phosphate in the GAC, as well as the serotype c carbohydrate from Streptococcus mutans, which depended on the presence of the respective gacH homologs. Finally, nuclear magnetic resonance analysis of GAC confirmed that glycerol phosphate is attached to approximately 25% of the GAC N-acetylglucosamine side-chains at the C6 hydroxyl group. This previously unrecognized structural modification impacts host-pathogen interaction and has implications for vaccine design.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Edgar, Rebecca J ...
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van Hensbergen, ...
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Ruda, Alessandro
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Turner, Andrew G ...
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Deng, Pan
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Le Breton, Yoann
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show more...
-
El-Sayed, Najib ...
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Belew, Ashton T.
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McIver, Kevin S.
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McEwan, Alastair ...
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Morris, Andrew J ...
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Lambeau, Gérard
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Walker, Markj
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Rush, Jeffrey S.
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Korotkov, Konsta ...
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Widmalm, Göran
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van Sorge, Nina ...
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Korotkova, Natal ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
- Articles in the publication
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Nature Chemical ...
- By the university
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Stockholm University