Distinct structural modulation of photosystem I and lipid environment stabilizes its tetrameric assembly

Chen, Ming (author): Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Tsinghua University, China

Perez-Boerema, Annemarie (author): Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Karolinska Institutet, Sweden

Zhang, Laixing (author)

Li, Yanxue (author)

Yang, Maojun (author)

Li, Shizhong (author)

Amunts, Alexey (author): Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Karolinska Institutet, Sweden

(creator_code:org_t)

2020-03-09
2020
English.
In: Nature Plants. - : Springer Science and Business Media LLC. - 2055-026X .- 2055-0278. ; 6:3, s. 314-

Related links:: https://urn.kb.se/re...; show more...; https://doi.org/10.1...; http://kipublication...; show less...

Abstract Subject headings

Photosystem I (PSI) is able to form different oligomeric states across various species. To reveal the structural basis for PSI dimerization and tetramerization, we structurally investigated PSI from the cyanobacterium Anabaena. This revealed a disrupted trimerization domain due to lack of the terminal residues of PsaL in the lumen, which resulted in PSI dimers with loose connections between monomers and weaker energy-coupled chlorophylls than in the trimer. At the dimer surface, specific phospholipids, cofactors and interactions in combination facilitated recruitment of another dimer to form a tetramer. Taken together, the relaxed luminal connections and lipid specificity at the dimer interface account for membrane curvature. PSI tetramer assembly appears to increase the surface area of the thylakoid membrane, which would contribute to PSI crowding. Photosystem I from the cyanobacterium Anabaena has a disrupted trimerization domain resulting in dimers with loose connections between monomers. Phospholipids and cofactors at the dimer surface facilitate further dimerization to form a tetramer.

By the author/editor: Chen, Ming; Perez-Boerema, A ...; Zhang, Laixing; Li, Yanxue; Yang, Maojun; Li, Shizhong; show more...; Amunts, Alexey; show less...

About the subject

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