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Dry Generation of C...
Dry Generation of CeO2 Nanoparticles and Deposition onto a Co-Culture of A549 and THP-1 Cells in Air-Liquid Interface-Dosimetry Considerations and Comparison to Submerged Exposure
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- Cappellini, Francesca (author)
- Karolinska Inst, Inst Environm Med, S-17177 Stockholm, Sweden.
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- Di Bucchianico, Sebastiano (author)
- Karolinska Inst, Inst Environm Med, S-17177 Stockholm, Sweden.;Helmholtz Zentrum Munchen, Comprehens Mol Analyt, D-81379 Munich, Germany.
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- Karri, Venkatanaidu (author)
- Karolinska Inst, Inst Environm Med, S-17177 Stockholm, Sweden.
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- Latvala, Siiri (author)
- Stockholms universitet,Institutionen för miljövetenskap,Stockholm Univ, Dept Environm Sci, S-11418 Stockholm, Sweden.
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- Malmlof, Maria (author)
- Karolinska Institutet
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- Kippler, Maria (author)
- Karolinska Inst, Inst Environm Med, S-17177 Stockholm, Sweden.
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- Elihn, Karine (author)
- Stockholms universitet,Institutionen för miljövetenskap,Stockholm Univ, Dept Environm Sci, S-11418 Stockholm, Sweden.
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- Hedberg, Jonas, 1979- (author)
- KTH,Yt- och korrosionsvetenskap
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- Odnevall Wallinder, Inger, 1965- (author)
- KTH,Yt- och korrosionsvetenskap
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- Gerde, Per (author)
- Karolinska Inst, Inst Environm Med, S-17177 Stockholm, Sweden.;Inhalat Sci, Halsovagen 7-9, S-14157 Huddinge, Sweden.
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- Karlsson, Hanna L. (author)
- Karolinska Institutet
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Karolinska Inst, Inst Environm Med, S-17177 Stockholm, Sweden Karolinska Inst, Inst Environm Med, S-17177 Stockholm, Sweden.;Helmholtz Zentrum Munchen, Comprehens Mol Analyt, D-81379 Munich, Germany. (creator_code:org_t)
- 2020-03-27
- 2020
- English.
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In: Nanomaterials. - : MDPI AG. - 2079-4991. ; 10:4
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Abstract
Subject headings
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- Relevant in vitro assays that can simulate exposure to nanoparticles (NPs) via inhalation are urgently needed. Presently, the most common method employed is to expose lung cells under submerged conditions, but the cellular responses to NPs under such conditions might differ from those observed at the more physiological air-liquid interface (ALI). The aim of this study was to investigate the cytotoxic and inflammatory potential of CeO2 NPs (NM-212) in a co-culture of A549 lung epithelial cells and differentiated THP-1 cells in both ALI and submerged conditions. Cellular dose was examined quantitatively using inductively coupled plasma mass spectrometry (ICP-MS). The role of serum and LPS-priming for IL-1 beta release was further tested in THP-1 cells in submerged exposure. An aerosol of CeO2 NPs was generated by using the PreciseInhale (R) system, and NPs were deposited on the co-culture using XposeALI (R). No or minor cytotoxicity and no increased release of inflammatory cytokines (IL-1 beta, IL-6, TNF alpha, MCP-1) were observed after exposure of the co-culture in ALI (max 5 mu g/cm(2)) or submerged (max 22 mu g/cm(2)) conditions. In contrast, CeO2 NPs cause clear IL-1 beta release in monocultures of macrophage-like THP-1, independent of the presence of serum and LPS-priming. This study demonstrates a useful approach for comparing effects at various in-vitro conditions.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Keyword
- nanotoxicology
- air-liquid interface
- PreciseInhale
- dosimetry
- inflammation
- ceria
Publication and Content Type
- ref (subject category)
- art (subject category)
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Cappellini, Fran ...
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Di Bucchianico, ...
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Karri, Venkatana ...
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Latvala, Siiri
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Malmlof, Maria
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Kippler, Maria
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Elihn, Karine
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Hedberg, Jonas, ...
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Odnevall Wallind ...
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Gerde, Per
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Karlsson, Hanna ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Pharmacology and ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Medical Biotechn ...
- Articles in the publication
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Nanomaterials
- By the university
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Stockholm University
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Royal Institute of Technology
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Karolinska Institutet