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Current status and ...
Current status and future opportunities for serial crystallography at MAX IV Laboratory
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- Shilova, Anastasiia (author)
- Lund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory,MAX IV Laboratory, Lund University, Lund, Sweden
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- Lebrette, Hugo (author)
- Stockholm University,Stockholms universitet,Institutionen för biokemi och biofysik,Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden
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- Aurelius, Oskar (author)
- Lund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory,MAX IV Laboratory, Lund University, Lund, Sweden
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- Nan, Jie (author)
- Lund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory
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- Welin, Martin (author)
- SARomics Biostructures AB
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- Kovacic, Rebeka (author)
- SARomics Biostructures AB
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- Ghosh, Swagatha, 1987 (author)
- Gothenburg University,Göteborgs universitet,University of Gothenburg,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Safari, Cecilia, 1989 (author)
- Gothenburg University,Göteborgs universitet,University of Gothenburg,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Friel, Ross J. (author)
- Högskolan i Halmstad,Halmstad University,Centrum för forskning om inbyggda system (CERES)
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- Milas, Mirko (author)
- Lund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory
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- Matej, Zdenek (author)
- Lund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory
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- Högbom, Martin (author)
- Stockholm University,Stockholms universitet,Institutionen för biokemi och biofysik
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- Brändén, Gisela, 1975 (author)
- Gothenburg University,Göteborgs universitet,University of Gothenburg,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Kloos, Marco (author)
- European XFEL GmbH
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- Shoeman, Robert L. (author)
- Max-Planck-Institut für Medizinische Forschung
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- Doak, Bruce (author)
- Max-Planck-Institut für Medizinische Forschung
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- Ursby, Thomas (author)
- Lund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory
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- Hakansson, Maria (author)
- SARomics Biostructures AB
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- Logan, Derek T. (author)
- SARomics Biostructures AB
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- Müller, Uwe (author)
- Lund University,Lunds universitet,MAX IV-laboratoriet,MAX IV Laboratory,Helmholtz Association of German Research Centers
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(creator_code:org_t)
- Chichester : Wiley-Blackwell, 2020
- 2020
- English.
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In: Journal of Synchrotron Radiation. - Chichester : Wiley-Blackwell. - 0909-0495 .- 1600-5775. ; 27, s. 1095-1102
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Abstract
Subject headings
Close
- Over the last decade, serial crystallography, a method to collect complete diffraction datasets from a large number of microcrystals delivered and exposed to an X-ray beam in random orientations at room temperature, has been successfully implemented at X-ray free-electron lasers and synchrotron radiation facility beamlines. This development relies on a growing variety of sample presentation methods, including different fixed target supports, injection methods using gas-dynamic virtual-nozzle injectors and high-viscosity extrusion injectors, and acoustic levitation of droplets, each with unique requirements. In comparison with X-ray free-electron lasers, increased beam time availability makes synchrotron facilities very attractive to perform serial synchrotron X-ray crystallography (SSX) experiments. Within this work, the possibilities to perform SSX at BioMAX, the first macromolecular crystallography beamline at studies from the SSX user program: an implementation of a high-viscosity extrusion injector to perform room temperature serial crystallography at BioMAX using two solid supports - silicon nitride membranes (Silson, UK) and XtalTool (Jena Bioscience, Germany). Future perspectives for the dedicated serial crystallography beamline MicroMAX at MAX IV Laboratory, which will provide parallel and intense micrometre-sized X-ray beams, are discussed.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
Keyword
- serial crystallography
- macromolecular crystallography
- sample delivery
- high-viscosity injectors
- high-viscosity injectors
- macromolecular crystallography
- sample delivery
- serial crystallography
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Shilova, Anastas ...
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Lebrette, Hugo
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Aurelius, Oskar
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Nan, Jie
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Welin, Martin
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Kovacic, Rebeka
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show more...
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Ghosh, Swagatha, ...
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Safari, Cecilia, ...
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Friel, Ross J.
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Milas, Mirko
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Matej, Zdenek
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Högbom, Martin
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Brändén, Gisela, ...
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Kloos, Marco
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Shoeman, Robert ...
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Doak, Bruce
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Ursby, Thomas
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Hakansson, Maria
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Logan, Derek T.
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Müller, Uwe
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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and Atom and Molecul ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Structural Biolo ...
- Articles in the publication
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Journal of Synch ...
- By the university
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Stockholm University
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Lund University
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Halmstad University
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University of Gothenburg