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Single Cell RNA Exp...
Single Cell RNA Expression Analysis Using Flow Cytometry Based on Specific Probe Ligation and Rolling Circle Amplification
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Zhao, Yansong (author)
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Lin, Chen (author)
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Wu, Pengcheng (author)
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Chen, Xiaoyuan (author)
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Zhao, Yuancun (author)
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Li, Yupeng (author)
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Chen, Lu (author)
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- Nilsson, Mats (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab)
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Ke, Rongqin (author)
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(creator_code:org_t)
- 2020-09-16
- 2020
- English.
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In: ACS Sensors. - : American Chemical Society (ACS). - 2379-3694. ; 5:10, s. 3031-3036
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Conventional flow cytometry has been widely used for high-throughput single-cell gene expression analysis using specific antibody staining. However, this is limited by the availability of high-quality antibodies. We developed a novel flow cytometry RNA detection technique termed RCA-Flow for single-cell RNA expression analysis. We showed that it is able to analyze not only mRNAs but also microRNAs and circular RNAs that are otherwise difficult to analyze by other flow cytometry techniques. The versatility for high-throughput analysis of different types of RNA molecules makes our method possess great potential for both biomedical and clinical applications.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- flow cytometry
- single cell
- rolling circle amplification
- RNA expression
- microRNA
- circular RNA
Publication and Content Type
- ref (subject category)
- art (subject category)
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