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- Zhao, Yansong (author)
Single Cell RNA Expression Analysis Using Flow Cytometry Based on Specific Probe Ligation and Rolling Circle Amplification
- Article/chapterEnglish2020
Publisher, publication year, extent ...
- 2020-09-16
- American Chemical Society (ACS),2020
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:su-188906
- https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-188906URI
- https://doi.org/10.1021/acssensors.0c01569DOI
Supplementary language notes
- Language:English
- Summary in:English
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- SwePubSwePub
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Notes
- Conventional flow cytometry has been widely used for high-throughput single-cell gene expression analysis using specific antibody staining. However, this is limited by the availability of high-quality antibodies. We developed a novel flow cytometry RNA detection technique termed RCA-Flow for single-cell RNA expression analysis. We showed that it is able to analyze not only mRNAs but also microRNAs and circular RNAs that are otherwise difficult to analyze by other flow cytometry techniques. The versatility for high-throughput analysis of different types of RNA molecules makes our method possess great potential for both biomedical and clinical applications.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
- flow cytometry
- single cell
- rolling circle amplification
- RNA expression
- microRNA
- circular RNA
Added entries (persons, corporate bodies, meetings, titles ...)
- Lin, Chen (author)
- Wu, Pengcheng (author)
- Chen, Xiaoyuan (author)
- Zhao, Yuancun (author)
- Li, Yupeng (author)
- Chen, Lu (author)
- Nilsson, MatsStockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab)(Swepub:su)matsn (author)
- Ke, Rongqin (author)
- Stockholms universitetInstitutionen för biokemi och biofysik (creator_code:org_t)
Related titles
- In:ACS Sensors: American Chemical Society (ACS)5:10, s. 3031-30362379-3694
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