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  • Acevedo, Nathalie (author)

Epigenetic alterations in skin homing CD4(+)CLA(+) T cells of atopic dermatitis patients

  • Article/chapterEnglish2020

Publisher, publication year, extent ...

  • 2020-10-22
  • Springer Science and Business Media LLC,2020
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:su-191268
  • https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-191268URI
  • https://doi.org/10.1038/s41598-020-74798-zDOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:145805558URI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • T cells expressing the cutaneous lymphocyte antigen (CLA) mediate pathogenic inflammation in atopic dermatitis (AD). The molecular alterations contributing to their dysregulation remain unclear. With the aim to elucidate putative altered pathways in AD we profiled DNA methylation levels and miRNA expression in sorted T cell populations -(CD4(+), -CD4(+)CD45RA(+) naive, -CD4(+)CLA(+), and -CD8(+)) from adult AD patients and healthy controls (HC). Skin homing -CD4(+)CLA(+) T cells from AD patients showed significant differences in DNA methylation in 40 genes compared to HC (p < 0.05). Reduced DNA methylation levels in the upstream region of the interleukin-13 gene (IL13) in -CD4(+)CLA(+) T cells from AD patients correlated with increased IL13 mRNA expression in these cells. Sixteen miRNAs showed differential expression in -CD4(+)CLA(+) T cells from AD patients targeting genes in 202 biological processes (p < 0.05). An integrated network analysis of miRNAs and CpG sites identified two communities of strongly interconnected regulatory elements with strong antagonistic behaviours that recapitulated the differences between AD patients and HC. Functional analysis of the genes linked to these communities revealed their association with key cytokine signaling pathways, MAP kinase signaling and protein ubiquitination. Our findings support that epigenetic mechanisms play a role in the pathogenesis of AD by affecting inflammatory signaling molecules in skin homing -CD4(+)CLA(+) T cells and uncover putative molecules participating in AD pathways.

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  • Benfeitas, RuiStockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab)(Swepub:su)rube5211 (author)
  • Katayama, ShintaroKarolinska Institutet (author)
  • Bruhn, Sören (author)
  • Andersson, AnnaKarolinska Institutet (author)
  • Wikberg, Gustav (author)
  • Lundeberg, Lena (author)
  • Lindvall, Jessica M.Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab)(Swepub:su)jeli1689 (author)
  • Greco, Dario (author)
  • Kere, JuhaKarolinska Institutet (author)
  • Söderhäll, CillaKarolinska Institutet (author)
  • Scheynius, Annika (author)
  • Stockholms universitetInstitutionen för biokemi och biofysik (creator_code:org_t)

Related titles

  • In:Scientific Reports: Springer Science and Business Media LLC10:12045-2322

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