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Identification and ...
Identification and characterization of distinct brown adipocyte subtypes in C57BL/6J mice
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Karlina, Ruth (author)
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Lutter, Dominik (author)
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Miok, Viktorian (author)
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Fischer, David (author)
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Altun, Irem (author)
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Schöttl, Theresa (author)
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Schorpp, Kenji (author)
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Israel, Andreas (author)
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Cero, Cheryl (author)
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Johnson, James W. (author)
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Kapser-Fischer, Ingrid (author)
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Böttcher, Anika (author)
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- Keipert, Susanne (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
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Feuchtinger, Annette (author)
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Graf, Elisabeth (author)
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Strom, Tim (author)
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Walch, Axel (author)
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Lickert, Heiko (author)
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Walzthoeni, Thomas (author)
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Heinig, Matthias (author)
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Theis, Fabian J. (author)
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García-Cáceres, Cristina (author)
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Cypess, Aaron M. (author)
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Ussar, Siegfried (author)
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(creator_code:org_t)
- 2020-11-30
- 2021
- English.
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In: Life Science Alliance. - : Life Science Alliance, LLC. - 2575-1077. ; 4:1
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https://doi.org/10.2...
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Abstract
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- Brown adipose tissue (BAT) plays an important role in the regulation of body weight and glucose homeostasis. Although increasing evidence supports white adipose tissue heterogeneity, little is known about heterogeneity within murine BAT. Recently, UCP1 high and low expressing brown adipocytes were identified, but a developmental origin of these subtypes has not been studied. To obtain more insights into brown preadipocyte heterogeneity, we use single-cell RNA sequencing of the BAT stromal vascular fraction of C57/BL6 mice and characterize brown preadipocyte and adipocyte clonal cell lines. Statistical analysis of gene expression profiles from brown preadipocyte and adipocyte clones identify markers distinguishing brown adipocyte subtypes. We confirm the presence of distinct brown adipocyte populations in vivo using the markers EIF5, TCF25, and BIN1. We also demonstrate that loss of Bin1 enhances UCP1 expression and mitochondrial respiration, suggesting that BIN1 marks dormant brown adipocytes. The existence of multiple brown adipocyte subtypes suggests distinct functional properties of BAT depending on its cellular composition, with potentially distinct functions in thermogenesis and the regulation of whole body energy homeostasis.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Karlina, Ruth
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Lutter, Dominik
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Miok, Viktorian
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Fischer, David
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Altun, Irem
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Schöttl, Theresa
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show more...
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Schorpp, Kenji
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Israel, Andreas
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Cero, Cheryl
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Johnson, James W ...
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Kapser-Fischer, ...
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Böttcher, Anika
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Keipert, Susanne
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Feuchtinger, Ann ...
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Graf, Elisabeth
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Strom, Tim
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Walch, Axel
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Lickert, Heiko
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Walzthoeni, Thom ...
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Heinig, Matthias
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Theis, Fabian J.
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García-Cáceres, ...
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Cypess, Aaron M.
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Ussar, Siegfried
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
- Articles in the publication
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Life Science All ...
- By the university
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Stockholm University