Search: onr:"swepub:oai:DiVA.org:su-193390" >
CRISPR-Mediated Ind...
-
Russo, Gianluca L.
(author)
CRISPR-Mediated Induction of Neuron-Enriched Mitochondrial Proteins Boosts Direct Glia-to-Neuron Conversion
- Article/chapterEnglish2021
Publisher, publication year, extent ...
-
Elsevier BV,2021
-
printrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:su-193390
-
https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-193390URI
-
https://doi.org/10.1016/j.stem.2020.10.015DOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
Astrocyte-to-neuron conversion is a promising avenue for neuronal replacement therapy. Neurons are particularly dependent on mitochondrial function, but how well mitochondria adapt to the new fate is unknown. Here, we determined the comprehensive mitochondrial proteome of cortical astrocytes and neurons, identifying about 150 significantly enriched mitochondrial proteins for each cell type, including transporters, metabolic enzymes, and cell-type-specific antioxidants. Monitoring their transition during reprogramming revealed late and only partial adaptation to the neuronal identity. Early dCas9-mediated activation of genes encoding mitochondrial proteins significantly improved conversion efficiency, particularly for neuron-enriched but not astrocyte-enriched antioxidant proteins. For example, Sod1 not only improves the survival of the converted neurons but also elicits a faster conversion pace, indicating that mitochondrial proteins act as enablers and drivers in this process. Transcriptional engineering of mitochondrial proteins with other functions improved reprogramming as well, demonstrating a broader role of mitochondrial proteins during fate conversion.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Sonsalla, Giovanna
(author)
-
Natarajan, Poornemaa
(author)
-
Breunig, Christopher T.
(author)
-
Bulli, Giorgia
(author)
-
Merl-Pham, Juliane
(author)
-
Schmitt, Sabine
(author)
-
Giehrl-Schwab, Jessica
(author)
-
Giesert, Florian
(author)
-
Jastroch, MartinStockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut(Swepub:su)maja7434
(author)
-
Zischka, Hans
(author)
-
Wurst, Wolfgang
(author)
-
Stricker, Stefan H.
(author)
-
Hauck, Stefanie M.
(author)
-
Masserdotti, Giacomo
(author)
-
Götz, Magdalena
(author)
-
Stockholms universitetInstitutionen för molekylär biovetenskap, Wenner-Grens institut
(creator_code:org_t)
Related titles
-
In:Cell Stem Cell: Elsevier BV28:3, s. 524-5341934-59091875-9777
Internet link
Find in a library
To the university's database
- By the author/editor
-
Russo, Gianluca ...
-
Sonsalla, Giovan ...
-
Natarajan, Poorn ...
-
Breunig, Christo ...
-
Bulli, Giorgia
-
Merl-Pham, Julia ...
-
show more...
-
Schmitt, Sabine
-
Giehrl-Schwab, J ...
-
Giesert, Florian
-
Jastroch, Martin
-
Zischka, Hans
-
Wurst, Wolfgang
-
Stricker, Stefan ...
-
Hauck, Stefanie ...
-
Masserdotti, Gia ...
-
Götz, Magdalena
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Neurosciences
- Articles in the publication
-
Cell Stem Cell
- By the university
-
Stockholm University