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  • Clabbers, Max T. B.Stockholms universitet,Institutionen för material- och miljökemi (MMK) (author)

MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography

  • Article/chapterEnglish2021

Publisher, publication year, extent ...

  • 2021-05-10
  • Springer Science and Business Media LLC,2021
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:su-196789
  • https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-196789URI
  • https://doi.org/10.1038/s41467-021-22590-6DOI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • MyD88 and MAL are Toll-like receptor (TLR) adaptors that signal to induce pro-inflammatory cytokine production. We previously observed that the TIR domain of MAL (MALTIR) forms filaments in vitro and induces formation of crystalline higher-order assemblies of the MyD88 TIR domain (MyD88TIR). These crystals are too small for conventional X-ray crystallography, but are ideally suited to structure determination by microcrystal electron diffraction (MicroED) and serial femtosecond crystallography (SFX). Here, we present MicroED and SFX structures of the MyD88TIR assembly, which reveal a two-stranded higher-order assembly arrangement of TIR domains analogous to that seen previously for MALTIR. We demonstrate via mutagenesis that the MyD88TIR assembly interfaces are critical for TLR4 signaling in vivo, and we show that MAL promotes unidirectional assembly of MyD88TIR. Collectively, our studies provide structural and mechanistic insight into TLR signal transduction and allow a direct comparison of the MicroED and SFX techniques.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Holmes, Susannah (author)
  • Muusse, Timothy W. (author)
  • Vajjhala, Parimala R. (author)
  • Thygesen, Sara J. (author)
  • Malde, Alpeshkumar K. (author)
  • Hunter, Dominic J. B. (author)
  • Croll, Tristan (author)
  • Flueckiger, Leonie (author)
  • Nanson, Jeffrey D. (author)
  • Rahaman, Md Habibur (author)
  • Aquila, Andrew (author)
  • Hunter, Mark S. (author)
  • Liang, Mengning (author)
  • Yoon, Chun Hong (author)
  • Zhao, JingjingStockholms universitet,Institutionen för material- och miljökemi (MMK)(Swepub:su)jinzh (author)
  • Zatsepin, Nadia A. (author)
  • Abbey, Brian (author)
  • Sierecki, Emma (author)
  • Gambin, Yann (author)
  • Stacey, Katryn J. (author)
  • Darmanin, Connie (author)
  • Kobe, Bostjan (author)
  • Xu, HongyiStockholms universitet,Institutionen för material- och miljökemi (MMK)(Swepub:su)hxu (author)
  • Ve, Thomas (author)
  • Stockholms universitetInstitutionen för material- och miljökemi (MMK) (creator_code:org_t)

Related titles

  • In:Nature Communications: Springer Science and Business Media LLC12:12041-1723

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