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Rapid Identificatio...
Rapid Identification of Common Secondary Metabolites of Medicinal Herbs Using High-Performance Liquid Chromatography with Evaporative Light Scattering Detector in Extracts
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Ali, Kiran (author)
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Ali, Arslan (author)
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Noman Khan, Muhammad (author)
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Rahman, Saeedur (author)
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Faizi, Shaheen (author)
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Ali, Muhammad Shaiq (author)
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- Khalifa, Shaden A. M. (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
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- El-Seedi, Hesham R. (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,Menoufia University, Egypt; Jiangsu University, China
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Musharraf, Syed Ghulam (author)
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(creator_code:org_t)
- 2021-07-28
- 2021
- English.
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In: Metabolites. - : MDPI AG. - 2218-1989 .- 2218-1989. ; 11:8
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https://doi.org/10.3...
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https://www.mdpi.com...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- The discovery and identification of novel natural products of medicinal importance in the herbal medicine industry becomes a challenge. The complexity of this process can be reduced by dereplication strategies. The current study includes a method based on high-performance liquid chromatography (HPLC), using the evaporative light scattering detector (ELSD) to identify the 12 most common secondary metabolites in plant extracts. Twelve compounds including rutin, taxifolin, quercetin, apigenin, kaempferol, betulinic acid, oleanolic acid, betulin, lupeol, stigmasterol, and beta-sitosterol were analyzed simultaneously. The polarity of the compounds varied greatly from highly polar (flavonoids) to non-polar (triterpenes and sterols). This method was also tested for HPLC-DAD and HPLC-ESI-MS/MS analysis. Oleanolic acid and ursolic acid could not be separated in HPLC-ELSD analysis but were differentiated using LC-ESI-MS/MS analysis due to different fragment ions. The regression values (R-2 > 0.996) showed good linearity in the range of 50-1000 mu g/mL for all compounds. The range of LOD and LOQ values were 7.76-38.30 mu g/mL and 23.52-116.06 mu g/mL, respectively. %RSD and % trueness values of inter and intraday studies were mostly <10%. This method was applied on 10 species of medicinal plants. The dereplication strategy has the potential to facilitate and shorten the identification process of common secondary metabolites in complex plant extracts.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- dereplication
- HPLC-ELSD
- plant extracts
- flavonoids
- triterpenes
- sterols
Publication and Content Type
- ref (subject category)
- art (subject category)
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