Search: onr:"swepub:oai:DiVA.org:su-198440" >
A simple pressure-a...
A simple pressure-assisted method for MicroED specimen preparation
-
- Zhao, Jingjing (author)
- Stockholms universitet,Institutionen för material- och miljökemi (MMK)
-
- Xu, Hongyi (author)
- Stockholms universitet,Institutionen för material- och miljökemi (MMK)
-
- Lebrette, Hugo (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
-
show more...
-
- Carroni, Marta (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab)
-
Taberman, Helena (author)
-
- Högbom, Martin (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
-
- Zou, Xiaodong (author)
- Stockholms universitet,Institutionen för material- och miljökemi (MMK)
-
show less...
-
(creator_code:org_t)
- 2021-08-19
- 2021
- English.
-
In: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 12:1
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://www.nature.c...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Micro-crystal electron diffraction (MicroED) has shown great potential for structure determination of macromolecular crystals too small for X-ray diffraction. However, specimen preparation remains a major bottleneck. Here, we report a simple method for preparing MicroED specimens, named Preassis, in which excess liquid is removed through an EM grid with the assistance of pressure. We show the ice thicknesses can be controlled by tuning the pressure in combination with EM grids with appropriate carbon hole sizes. Importantly, Preassis can handle a wide range of protein crystals grown in various buffer conditions including those with high viscosity, as well as samples with low crystal concentrations. Preassis is a simple and universal method for MicroED specimen preparation, and will significantly broaden the applications of MicroED.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database