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Action spectroscopy...
Action spectroscopy of the isolated red Kaede fluorescent protein chromophore
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Coughlan, Neville J. A. (author)
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- Stockett, Mark H. (author)
- Stockholms universitet,Fysikum
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Kjær, Christina (author)
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Ashworth, Eleanor K. (author)
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Bulman Page, Philip C. (author)
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Meech, Stephen R. (author)
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Brøndsted Nielsen, Steen (author)
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Blancafort, Lluís (author)
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Hopkins, W. Scott (author)
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Bull, James N. (author)
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(creator_code:org_t)
- AIP Publishing, 2021
- 2021
- English.
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In: Journal of Chemical Physics. - : AIP Publishing. - 0021-9606 .- 1089-7690. ; 155:12
- Related links:
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https://ueaeprints.u...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Incorporation of fluorescent proteins into biochemical systems has revolutionized the field of bioimaging. In a bottom-up approach, understanding the photophysics of fluorescent proteins requires detailed investigations of the light-absorbing chromophore, which can be achieved by studying the chromophore in isolation. This paper reports a photodissociation action spectroscopy study on the deprotonated anion of the red Kaede fluorescent protein chromophore, demonstrating that at least three isomers–assigned to deprotomers–are generated in the gas phase. Deprotomer-selected action spectra are recorded over the S1 ← S0 band using an instrument with differential mobility spectrometry coupled with photodissociation spectroscopy. The spectrum for the principal phenoxide deprotomer spans the 480–660 nm range with a maximum response at ≈610 nm. The imidazolate deprotomer has a blue-shifted action spectrum with a maximum response at ≈545 nm. The action spectra are consistent with excited state coupled-cluster calculations of excitation wavelengths for the deprotomers. A third gas-phase species with a distinct action spectrum is tentatively assigned to an imidazole tautomer of the principal phenoxide deprotomer. This study highlights the need for isomer-selective methods when studying the photophysics of biochromophores possessing several deprotonation sites.
Subject headings
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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