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PplD is a de-N-acet...
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Rush, Jeffrey S.
(author)
PplD is a de-N-acetylase of the cell wall linkage unit of streptococcal rhamnopolysaccharides
- Article/chapterEnglish2022
Publisher, publication year, extent ...
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2022-02-01
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Springer Science and Business Media LLC,2022
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:su-202295
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-202295URI
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https://doi.org/10.1038/s41467-022-28257-0DOI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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The cell wall of the human bacterial pathogen Group A Streptococcus (GAS) consists of peptidoglycan decorated with the Lancefield group A carbohydrate (GAC). GAC is a promising target for the development of GAS vaccines. In this study, employing chemical, compositional, and NMR methods, we show that GAC is attached to peptidoglycan via glucosamine 1-phosphate. This structural feature makes the GAC-peptidoglycan linkage highly sensitive to cleavage by nitrous acid and resistant to mild acid conditions. Using this characteristic of the GAS cell wall, we identify PplD as a protein required for deacetylation of linkage N-acetylglucosamine (GlcNAc). X-ray structural analysis indicates that PplD performs catalysis via a modified acid/base mechanism. Genetic surveys in silico together with functional analysis indicate that PplD homologs deacetylate the polysaccharide linkage in many streptococcal species. We further demonstrate that introduction of positive charges to the cell wall by GlcNAc deacetylation protects GAS against host cationic antimicrobial proteins.
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Parajuli, Prakash
(author)
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Ruda, Alessandro,1990-Stockholms universitet,Institutionen för organisk kemi(Swepub:su)alru3031
(author)
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Li, Jian
(author)
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Pohane, Amol Arunrao
(author)
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Zamakhaeva, Svetlana
(author)
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Rahman, Mohammad M.
(author)
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Chang, Jennifer C.
(author)
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Gogos, Artemis
(author)
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Kenner, Cameron W.
(author)
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Lambeau, Gérard
(author)
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Federle, Michael J.
(author)
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Korotkov, Konstantin
(author)
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Widmalm, GöranStockholms universitet,Institutionen för organisk kemi(Swepub:su)gw
(author)
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Korotkova, Natalia
(author)
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Stockholms universitetInstitutionen för organisk kemi
(creator_code:org_t)
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In:Nature Communications: Springer Science and Business Media LLC13:12041-1723
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Rush, Jeffrey S.
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Parajuli, Prakas ...
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Ruda, Alessandro ...
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Li, Jian
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Pohane, Amol Aru ...
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Zamakhaeva, Svet ...
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Rahman, Mohammad ...
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Chang, Jennifer ...
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Gogos, Artemis
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Kenner, Cameron ...
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Lambeau, Gérard
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Federle, Michael ...
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Korotkov, Konsta ...
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Widmalm, Göran
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Korotkova, Natal ...
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Stockholm University