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Patatin-like phosph...
Patatin-like phospholipase CapV in Escherichia coli-morphological and physiological effects of one amino acid substitution
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Li, Fengyang (author)
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Cao, Lianying (author)
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Bähre, Heike (author)
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Kim, Soo-Kyoung (author)
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- Schroeder, Kristen, 1986- (author)
- Stockholms universitet,Science for Life Laboratory (SciLifeLab),Institutionen för molekylär biovetenskap, Wenner-Grens institut
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- Jonas, Kristina (author)
- Stockholms universitet,Science for Life Laboratory (SciLifeLab),Institutionen för molekylär biovetenskap, Wenner-Grens institut
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- Koonce, Kira (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,Science for Life Laboratory (SciLifeLab)
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Mekonnen, Solomon A. (author)
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- Mohanty, Soumitra (author)
- Karolinska Institutet
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Bai, Fengwu (author)
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- Brauner, Annelie (author)
- Karolinska Institutet
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Lee, Vincent T. (author)
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Rohde, Manfred (author)
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- Römling, Ute (author)
- Karolinska Institutet
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(creator_code:org_t)
- 2022-05-11
- 2022
- English.
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In: npj Biofilms and Microbiomes. - : Springer Science and Business Media LLC. - 2055-5008. ; 8:1
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
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- In rod-shaped bacteria, morphological plasticity occurs in response to stress, which blocks cell division to promote filamentation. We demonstrate here that overexpression of the patatin-like phospholipase variant CapV(Q329R), but not CapV, causes pronounced sulA-independent pyridoxine-inhibited cell filamentation in the Escherichia coli K-12-derivative MG1655 associated with restriction of flagella production and swimming motility. Conserved amino acids in canonical patatin-like phospholipase A motifs, but not the nucleophilic serine, are required to mediate CapV(Q329R) phenotypes. Furthermore, CapV(Q329R) production substantially alters the lipidome and colony morphotype including rdar biofilm formation with modulation of the production of the biofilm activator CsgD, and affects additional bacterial traits such as the efficiency of phage infection and antimicrobial susceptibility. Moreover, genetically diverse commensal and pathogenic E. coli strains and Salmonella typhimurium responded with cell filamentation and modulation in colony morphotype formation to CapV(Q329R) expression. In conclusion, this work identifies the CapV variant CapV(Q329R) as a pleiotropic regulator, emphasizes a scaffold function for patatin-like phospholipases, and highlights the impact of the substitution of a single conserved amino acid for protein functionality and alteration of host physiology.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Li, Fengyang
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Cao, Lianying
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Bähre, Heike
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Kim, Soo-Kyoung
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Schroeder, Krist ...
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Jonas, Kristina
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show more...
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Koonce, Kira
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Mekonnen, Solomo ...
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Mohanty, Soumitr ...
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Bai, Fengwu
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Brauner, Annelie
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Lee, Vincent T.
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Rohde, Manfred
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Römling, Ute
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Microbiology in ...
- Articles in the publication
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npj Biofilms and ...
- By the university
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Stockholm University
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Karolinska Institutet