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Redox-controlled re...
Redox-controlled reorganization and flavin strain within the ribonucleotide reductase R2b–NrdI complex monitored by serial femtosecond crystallography
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- John, Juliane (author)
- Stockholm University,Stockholms universitet,Institutionen för biokemi och biofysik
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- Aurelius, Oskar (author)
- Stockholm University,Lunds universitet,Stockholms universitet,Institutionen för biokemi och biofysik,Lund University, Sweden,MAX IV-laboratoriet,MAX IV Laboratory
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- Srinivas, Vivek, 1988- (author)
- Stockholm University,Stockholms universitet,Institutionen för biokemi och biofysik
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- Saura, Patricia (author)
- Stockholm University,Stockholms universitet,Institutionen för biokemi och biofysik
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- Kim, In-Sik (author)
- Lawrence Berkeley National Laboratory
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- Bhowmick, Asmit (author)
- Lawrence Berkeley National Laboratory
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- Simon, Philipp S. (author)
- Lawrence Berkeley National Laboratory
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- Dasgupta, Medhanjali (author)
- Lawrence Berkeley National Laboratory
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- Pham, Cindy (author)
- Lawrence Berkeley National Laboratory
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- Gul, Sheraz (author)
- Lawrence Berkeley National Laboratory
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- Sutherlin, Kyle D. (author)
- Lawrence Berkeley National Laboratory
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- Aller, Pierre (author)
- Research Complex at Harwell,Diamond Light Source
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- Butryn, Agata (author)
- Research Complex at Harwell,Diamond Light Source
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- Orville, Allen M. (author)
- Diamond Light Source,Research Complex at Harwell
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- Cheah, Mun Hon (author)
- Uppsala University
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- Owada, Shigeki (author)
- Japan Synchrotron Radiation Research Institute,RIKEN SPring-8 center
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- Tono, Kensuke (author)
- RIKEN SPring-8 center,Japan Synchrotron Radiation Research Institute
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- Fuller, Franklin D. (author)
- Stanford Linear Accelerator Center (SLAC)
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- Batyuk, Alexander (author)
- Stanford Linear Accelerator Center (SLAC)
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- Brewster, Aaron S. (author)
- Lawrence Berkeley National Laboratory
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- Sauter, Nicholas K. (author)
- Lawrence Berkeley National Laboratory
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- Yachandra, Vittal K. (author)
- Lawrence Berkeley National Laboratory
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- Yano, Junko (author)
- Lawrence Berkeley National Laboratory
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- Kaila, Ville R. I. (author)
- Stockholm University,Stockholms universitet,Institutionen för biokemi och biofysik
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- Kern, Jan (author)
- Lawrence Berkeley National Laboratory
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- Lebrette, Hugo (author)
- Stockholm University,Stockholms universitet,Institutionen för biokemi och biofysik
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- Högbom, Martin (author)
- Stockholm University,Stockholms universitet,Institutionen för biokemi och biofysik
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(creator_code:org_t)
- 2022
- 2022
- English.
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In: eLIFE. - 2050-084X. ; 11
- Related links:
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https://doi.org/10.7...
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http://dx.doi.org/10... (free)
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https://urn.kb.se/re...
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https://doi.org/10.7...
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https://lup.lub.lu.s...
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Abstract
Subject headings
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- Redox reactions are central to biochemistry and are both controlled by and induce protein structural changes. Here, we describe structural rearrangements and crosstalk within the Bacillus cereus ribonucleotide reductase R2b–NrdI complex, a di-metal carboxylate-flavoprotein system, as part of the mechanism generating the essential catalytic free radical of the enzyme. Femtosecond crystallography at an X-ray free electron laser was utilized to obtain structures at room temperature in defined redox states without suffering photoreduction. Together with density functional theory calculations, we show that the flavin is under steric strain in the R2b–NrdI protein complex, likely tuning its redox properties to promote superoxide generation. Moreover, a binding site in close vicinity to the expected flavin O2 interaction site is observed to be controlled by the redox state of the flavin and linked to the channel proposed to funnel the produced superoxide species from NrdI to the di-manganese site in protein R2b. These specific features are coupled to further structural changes around the R2b–NrdI interaction surface. The mechanistic implications for the control of reactive oxygen species and radical generation in protein R2b are discussed.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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eLIFE
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To the university's database
- By the author/editor
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John, Juliane
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Aurelius, Oskar
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Srinivas, Vivek, ...
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Saura, Patricia
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Kim, In-Sik
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Bhowmick, Asmit
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show more...
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Simon, Philipp S ...
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Dasgupta, Medhan ...
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Pham, Cindy
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Gul, Sheraz
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Sutherlin, Kyle ...
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Aller, Pierre
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Butryn, Agata
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Orville, Allen M ...
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Cheah, Mun Hon
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Owada, Shigeki
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Tono, Kensuke
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Fuller, Franklin ...
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Batyuk, Alexande ...
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Brewster, Aaron ...
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Sauter, Nicholas ...
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Yachandra, Vitta ...
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Yano, Junko
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Kaila, Ville R. ...
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Kern, Jan
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Lebrette, Hugo
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Högbom, Martin
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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eLIFE
- By the university
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Stockholm University
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Lund University