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  • Ullah, HammadUniv Napoli Federico II, Dept Pharm, Via D Montesano 49, I-80131 Naples, Italy. (author)

In Vitro Bioaccessibility and Anti-Inflammatory Activity of a Chemically Characterized Allium cepa L. Extract Rich in Quercetin Derivatives Optimized by the Design of Experiments

  • Article/chapterEnglish2022

Publisher, publication year, extent ...

  • 2022-12-19
  • MDPI AG,2022
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:su-214530
  • https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-214530URI
  • https://doi.org/10.3390/molecules27249065DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-493390URI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Allium cepa L. is a highly consumed garden crop rich in biologically active phenolic and organosulfur compounds. This study aimed to assess the in vitro bioaccessibility and anti-inflammatory effect of a chemically characterized A. cepa extract rich in quercetin and its derivatives. Different varieties of A. cepa were studied; based on the highest total phenolic content, the “Golden” variety was selected. Its extracts, obtained from the tunicate bulb, tunic, and bulb, were subjected to determination of quercetin and its derivatives with LC-MS analysis and based on the highest total quercetin content, the tunic extract was utilized for further experiments. The extraction method was optimized through a design of experiment (DoE) method via full factorial design, which showed that 40% ethanol and 1 g tunic/20 mL solvent are the best extraction conditions. HPLC analysis of the optimized tunic extract identified 14 flavonols, including 10 quercetin derivatives. As far as in vitro bioaccessibility was concerned, the increases in some quercetin derivatives following the gastro-duodenal digestion process support the bioaccessibility of these bioactive compounds. Moreover, the extract significantly inhibited the production of PGE2 in stimulated J774 cell lines, while no effects of the tunic extract were observed against the release of IL-1β, TNF-α, and nitrites. The study provided insights into the optimized extraction conditions to obtain an A. cepa tunic extract rich in bioavailable quercetin derivatives with significant anti-inflammatory effects against PGE2.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Di Minno, AlessandroUniv Napoli Federico II, Dept Pharm, Via D Montesano 49, I-80131 Naples, Italy.;CEINGE Biotecnol Avanzate, Via Gaetano Salvatore 486, I-80145 Naples, Italy. (author)
  • Santarcangelo, CristinaUniv Napoli Federico II, Dept Pharm, Via D Montesano 49, I-80131 Naples, Italy. (author)
  • Tantipongpiradet, AriyawanUniv Napoli Federico II, Dept Pharm, Via D Montesano 49, I-80131 Naples, Italy. (author)
  • Dacrema, MarcoUniv Napoli Federico II, Dept Pharm, Via D Montesano 49, I-80131 Naples, Italy. (author)
  • di Matteo, RitaUniv Napoli Federico II, Dept Pharm, Via D Montesano 49, I-80131 Naples, Italy. (author)
  • El-Seedi, HeshamUppsala universitet,Institutionen för farmaceutisk biovetenskap,Jiangsu Univ, Int Res Ctr Food Nutr & Safety, Zhenjiang 212013, Peoples R China.(Swepub:uu)heels173 (author)
  • Khalifa, Shaden A. M.Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,Stockholm Univ, Wenner Gren Inst, Dept Mol Biosci, SE-10691 Stockholm, Sweden.(Swepub:su)shkha (author)
  • Baldi, AlessandraUniv Napoli Federico II, Dept Pharm, Via D Montesano 49, I-80131 Naples, Italy. (author)
  • Rossi, AntoniettaUniv Napoli Federico II, Dept Pharm, Via D Montesano 49, I-80131 Naples, Italy. (author)
  • Daglia, MariaUniv Napoli Federico II, Dept Pharm, Via D Montesano 49, I-80131 Naples, Italy.;Jiangsu Univ, Int Res Ctr Food Nutr & Safety, Zhenjiang 212013, Peoples R China. (author)
  • Univ Napoli Federico II, Dept Pharm, Via D Montesano 49, I-80131 Naples, Italy.Univ Napoli Federico II, Dept Pharm, Via D Montesano 49, I-80131 Naples, Italy.;CEINGE Biotecnol Avanzate, Via Gaetano Salvatore 486, I-80145 Naples, Italy. (creator_code:org_t)

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  • In:Molecules: MDPI AG27:241431-51571420-3049

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