Search: onr:"swepub:oai:DiVA.org:su-216979" >
Cold acclimation an...
Cold acclimation and pioglitazone combined increase thermogenic capacity of brown and white adipose tissues but this does not translate into higher energy expenditure in mice
-
Galvão Valdivia, Luís Felipe (author)
-
Castro, Érique (author)
-
dos Santos Eichler, Rosangela Aparecida (author)
-
show more...
-
Moreno, Mayara Franzoi (author)
-
de Sousa, Érica (author)
-
Rodrigues Jardim, Giovanna Freitas (author)
-
Souza Peixoto, Álbert (author)
-
Moraes, Maria Nathália (author)
-
de Lauro Castrucci, Ana Maria (author)
-
- Nedergaard, Jan (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
-
- Petrovic, Natasa (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
-
Festuccia, William T. (author)
-
Reckziegel, Patricia (author)
-
show less...
-
(creator_code:org_t)
- 2023-03-01
- 2023
- English.
-
In: American Journal of Physiology. Endocrinology and Metabolism. - : American Physiological Society. - 0193-1849 .- 1522-1555. ; 324:4, s. E358-E373
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Cold acclimation and pharmacological peroxisome proliferator-activated receptor γ (PPARγ) activation have each earlier been shown to recruit brown adipose tissue (BAT) and beige adipocytes thermogenic machinery, enhancing uncoupling protein 1 (UCP1)-mediated thermogenic capacity. We here investigated whether cold acclimation and PPARγ agonism combined have additive effects in inducing brown and beige adipocytes UCP1 content and whether this translates into a higher thermogenic capacity and energy expenditure. C57BL/6J mice treated or not with pioglitazone (30 mg/kg/day) were maintained at 21°C or exposed to cold (7°C) for 15 days and evaluated for thermogenic capacity, energy expenditure and interscapular BAT (iBAT) and inguinal white adipose tissue (iWAT) mass, morphology, UCP1 content and gene expression, glucose uptake and oxygen consumption. Cold acclimation and PPARγ agonism combined synergistically increased iBAT and iWAT total UCP1 content and mRNA levels of the thermogenesis-related proteins PGC1a, CIDEA, FABP4, GYK, PPARa, LPL, GLUTs (GLUT1 in iBAT and GLUT4 in iWAT), and ATG when compared to cold and pioglitazone individually. This translated into a stronger increase in body temperature in response to the β3-adrenergic agonist CL316,243 and iBAT and iWAT respiration induced by succinate and pyruvate in comparison to that seen in either cold-acclimated or pioglitazone-treated mice. However, basal energy expenditure, BAT glucose uptake and glucose tolerance were not increased above that seen in cold-acclimated untreated mice. In conclusion, cold acclimation and PPARγ agonism combined induced a robust increase in brown and beige adipocytes UCP1 content and thermogenic capacity, much higher than each treatment individually. However, our findings enforce the concept that increases in total UCP1 do not innately lead to higher energy expenditure.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- adipogenesis
- browning
- pioglitazone
- thermogenesis
- UCP1
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Galvão Valdivia, ...
-
Castro, Érique
-
dos Santos Eichl ...
-
Moreno, Mayara F ...
-
de Sousa, Érica
-
Rodrigues Jardim ...
-
show more...
-
Souza Peixoto, Á ...
-
Moraes, Maria Na ...
-
de Lauro Castruc ...
-
Nedergaard, Jan
-
Petrovic, Natasa
-
Festuccia, Willi ...
-
Reckziegel, Patr ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Physiology
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Cell and Molecul ...
- Articles in the publication
-
American Journal ...
- By the university
-
Stockholm University