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Oligomer Dynamics o...
Oligomer Dynamics of LL-37 Truncated Fragments Probed by α-Hemolysin Pore and Molecular Simulations
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Liu, Chang (author)
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Henning-Knechtel, Anja (author)
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- Österlund, Nicklas, 1992- (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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Wu, Jinming (author)
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Wang, Guangshun (author)
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- Gräslund, Rut Astrid Olivia, 1945- (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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Kirmizialtin, Serdal (author)
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Luo, Jinghui (author)
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(creator_code:org_t)
- 2023
- 2023
- English.
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In: Small. - 1613-6810 .- 1613-6829. ; 19:37
- Related links:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Oligomerization of antimicrobial peptides (AMPs) is critical in their effects on pathogens. LL-37 and its truncated fragments are widely investigated regarding their structures, antimicrobial activities, and application, such as developing new antibiotics. Due to the small size and weak intermolecular interactions of LL-37 fragments, it is still elusive to establish the relationship between oligomeric states and antimicrobial activities. Here, an α-hemolysin nanopore, mass spectrometry (MS), and molecular dynamic (MD) simulations are used to characterize the oligomeric states of two LL-37 fragments. Nanopore studies provide evidence of trapping events related to the oligomer formation and provide further details on their stabilities, which are confirmed by MS and MD simulations. Furthermore, simulation results reveal the molecular basis of oligomer dynamics and states of LL-37 fragments. This work provides unique insights into the relationship between the oligomer dynamics of AMPs and their antimicrobial activities at the single-molecule level. The study demonstrates how integrating methods allows deciphering single molecule level understanding from nanopore sensing approaches.
Subject headings
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Keyword
- dynamics
- LL-37 fragments
- nanopores
- oligomers
Publication and Content Type
- ref (subject category)
- art (subject category)
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