Search: onr:"swepub:oai:DiVA.org:su-221411" > Protomers of the gr...
Fältnamn | Indikatorer | Metadata |
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000 | 03120naa a2200421 4500 | |
001 | oai:DiVA.org:su-221411 | |
003 | SwePub | |
008 | 230920s2023 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-2214112 URI |
024 | 7 | a https://doi.org/10.1039/d3cp02661b2 DOI |
040 | a (SwePub)su | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Ashworth, Eleanor K.4 aut |
245 | 1 0 | a Protomers of the green and cyan fluorescent protein chromophores investigated using action spectroscopy |
264 | 1 | c 2023 |
338 | a print2 rdacarrier | |
520 | a The photophysics of biochromophore ions often depends on the isomeric or protomeric distribution, yet this distribution, and the individual isomer contributions to an action spectrum, can be difficult to quantify. Here, we use two separate photodissociation action spectroscopy instruments to record electronic spectra for protonated forms of the green (pHBDI(+)) and cyan (Cyan(+)) fluorescent protein chromophores. One instrument allows for cryogenic (T = 40 & PLUSMN; 10 K) cooling of the ions, while the other offers the ability to perform protomer-selective photodissociation spectroscopy. We show that both chromophores are generated as two protomers when using electrospray ionisation, and that the protomers have partially overlapping absorption profiles associated with the S-1 & LARR; S-0 transition. The action spectra for both species span the 340-460 nm range, although the spectral onset for the pHBDI(+) protomer with the proton residing on the carbonyl oxygen is red-shifted by & AP;40 nm relative to the lower-energy imine protomer. Similarly, the imine and carbonyl protomers are the lowest energy forms of Cyan(+), with the main band for the carbonyl protomer red-shifted by & AP;60 nm relative to the lower-energy imine protomer. The present strategy for investigating protomers can be applied to a wide range of other biochromophore ions. | |
650 | 7 | a NATURVETENSKAPx Kemi0 (SwePub)1042 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Chemical Sciences0 (SwePub)1042 hsv//eng |
650 | 7 | a NATURVETENSKAPx Fysik0 (SwePub)1032 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Physical Sciences0 (SwePub)1032 hsv//eng |
700 | 1 | a Dezalay, Jordanu Stockholms universitet,Fysikum4 aut0 (Swepub:su)jode5907 |
700 | 1 | a Ryan, Christopher R. M.4 aut |
700 | 1 | a Ieritano, Christian4 aut |
700 | 1 | a Hopkins, W. Scott4 aut |
700 | 1 | a Chambrier, Isabelle4 aut |
700 | 1 | a Cammidge, Andrew N.4 aut |
700 | 1 | a Stockett, Mark H.,d 1984-u Stockholms universitet,Fysikum4 aut0 (Swepub:su)mstoc |
700 | 1 | a Noble, Jennifer A.4 aut |
700 | 1 | a Bull, James N.4 aut |
710 | 2 | a Stockholms universitetb Fysikum4 org |
773 | 0 | t Physical Chemistry, Chemical Physics - PCCPg 25:30, s. 20405-20413q 25:30<20405-20413x 1463-9076x 1463-9084 |
856 | 4 | u https://doi.org/10.1039/d3cp02661by Fulltext |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-221411 |
856 | 4 8 | u https://doi.org/10.1039/d3cp02661b |
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