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Recombinant PreS-fusion protein vaccine for birch pollen and apple allergy

Khaitov, Musa (author)
Shilovskiy, Igor (author)
Valenta, Rudolf (author)
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Weber, Milena (author)
Korneev, Artem (author)
Tulaeva, Inna (author)
Gattinger, Pia (author)
van Hage, Marianne (author)
Karolinska Institutet
Hofer, Gerhard, 1985- (author)
Stockholms universitet,Institutionen för material- och miljökemi (MMK)
Konradsen, Jon R. (author)
Keller, Walter (author)
Akinfenwa, Oluwatoyin (author)
Poroshina, Alina (author)
Ilina, Nataliya (author)
Fedenko, Elena (author)
Elisyutina, Olga (author)
Litovkina, Alla (author)
Smolnikov, Evgenii (author)
Nikonova, Aleksandra (author)
Rybalkin, Sergei (author)
Aldobaev, Vladimir (author)
Smirnov, Valeriy (author)
Shershakova, Nadezhda (author)
Petukhova, Olga (author)
Kudlay, Dmitriy (author)
Shatilov, Artem (author)
Timofeeva, Anastasiya (author)
Campana, Raffaela (author)
Udin, Sergei (author)
Skvortsova, Veronica (author)
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 (creator_code:org_t)
2024
2024
English.
In: Allergy. European Journal of Allergy and Clinical Immunology. - 0105-4538 .- 1398-9995. ; 79:4, s. 1001-1017
Related links:
https://urn.kb.se/re...
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https://doi.org/10.1...
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  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Background: IgE cross-sensitization to major birch pollen allergen Bet v 1 and pathogenesis-related (PR10) plant food allergens is responsible for the pollen-food allergy syndrome.Methods: We designed a recombinant protein, AB-PreS, consisting of non-allergenic peptides derived from the IgE-binding sites of Bet v 1 and the cross-reactive apple allergen, Mal d 1, fused to the PreS domain of HBV surface protein as immunological carrier. AB-PreS was expressed in E. coli and purified by chromatography. The allergenic and inflammatory activity of AB-PreS was tested using basophils and PBMCs from birch pollen allergic patients. The ability of antibodies induced by immunization of rabbits with AB-PreS and birch pollen extract-based vaccines to inhibit allergic patients IgE binding to Bet v 1 and Mal d 1 was assessed by ELISA.Results: IgE-binding experiments and basophil activation test revealed the hypoallergenic nature of AB-PreS. AB-PreS induced lower T-cell activation and inflammatory cytokine production in cultured PBMCs from allergic patients. IgG antibodies induced by five injections with AB-PreS inhibited allergic patients' IgE binding to Bet v 1 and Mal d 1 better than did IgG induced by up to 30 injections of six licensed birch pollen allergen extract-based vaccines. Additionally, immunization with AB-PreS induced HBV-specific antibodies potentially protecting from infection with HBV.Conclusion: The recombinant AB-PreS-based vaccine is hypoallergenic and superior over currently registered allergen extract-based vaccines regarding the induction of blocking antibodies to Bet v 1 and Mal d 1 in animals.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Lungmedicin och allergi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Respiratory Medicine and Allergy (hsv//eng)

Keyword

allergen
allergen-specific immunotherapy
birch pollen-associated food allergy syndrome
molecular allergy vaccine

Publication and Content Type

ref (subject category)
art (subject category)

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