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Recombinant PreS-fu...
Recombinant PreS-fusion protein vaccine for birch pollen and apple allergy
- Article/chapterEnglish2024
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LIBRIS-ID:oai:DiVA.org:su-223748
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-223748URI
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https://doi.org/10.1111/all.15919DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:154055678URI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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Background: IgE cross-sensitization to major birch pollen allergen Bet v 1 and pathogenesis-related (PR10) plant food allergens is responsible for the pollen-food allergy syndrome.Methods: We designed a recombinant protein, AB-PreS, consisting of non-allergenic peptides derived from the IgE-binding sites of Bet v 1 and the cross-reactive apple allergen, Mal d 1, fused to the PreS domain of HBV surface protein as immunological carrier. AB-PreS was expressed in E. coli and purified by chromatography. The allergenic and inflammatory activity of AB-PreS was tested using basophils and PBMCs from birch pollen allergic patients. The ability of antibodies induced by immunization of rabbits with AB-PreS and birch pollen extract-based vaccines to inhibit allergic patients IgE binding to Bet v 1 and Mal d 1 was assessed by ELISA.Results: IgE-binding experiments and basophil activation test revealed the hypoallergenic nature of AB-PreS. AB-PreS induced lower T-cell activation and inflammatory cytokine production in cultured PBMCs from allergic patients. IgG antibodies induced by five injections with AB-PreS inhibited allergic patients' IgE binding to Bet v 1 and Mal d 1 better than did IgG induced by up to 30 injections of six licensed birch pollen allergen extract-based vaccines. Additionally, immunization with AB-PreS induced HBV-specific antibodies potentially protecting from infection with HBV.Conclusion: The recombinant AB-PreS-based vaccine is hypoallergenic and superior over currently registered allergen extract-based vaccines regarding the induction of blocking antibodies to Bet v 1 and Mal d 1 in animals.
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Shilovskiy, Igor
(author)
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Valenta, Rudolf
(author)
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Weber, Milena
(author)
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Korneev, Artem
(author)
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Tulaeva, Inna
(author)
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Gattinger, Pia
(author)
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van Hage, MarianneKarolinska Institutet
(author)
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Hofer, Gerhard,1985-Stockholms universitet,Institutionen för material- och miljökemi (MMK)(Swepub:su)geho3329
(author)
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Konradsen, Jon R.
(author)
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Keller, Walter
(author)
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Akinfenwa, Oluwatoyin
(author)
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Poroshina, Alina
(author)
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Ilina, Nataliya
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Fedenko, Elena
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Elisyutina, Olga
(author)
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Litovkina, Alla
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Smolnikov, Evgenii
(author)
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Nikonova, Aleksandra
(author)
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Rybalkin, Sergei
(author)
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Aldobaev, Vladimir
(author)
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Smirnov, Valeriy
(author)
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Shershakova, Nadezhda
(author)
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Petukhova, Olga
(author)
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Kudlay, Dmitriy
(author)
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Shatilov, Artem
(author)
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Timofeeva, Anastasiya
(author)
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Campana, Raffaela
(author)
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Udin, Sergei
(author)
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Skvortsova, Veronica
(author)
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Karolinska InstitutetInstitutionen för material- och miljökemi (MMK)
(creator_code:org_t)
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In:Allergy. European Journal of Allergy and Clinical Immunology79:4, s. 1001-10170105-45381398-9995
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Khaitov, Musa
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Shilovskiy, Igor
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Valenta, Rudolf
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Weber, Milena
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Korneev, Artem
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Tulaeva, Inna
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Gattinger, Pia
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van Hage, Marian ...
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Hofer, Gerhard, ...
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Konradsen, Jon R ...
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Keller, Walter
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Akinfenwa, Oluwa ...
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Poroshina, Alina
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Ilina, Nataliya
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Fedenko, Elena
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Elisyutina, Olga
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Litovkina, Alla
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Smolnikov, Evgen ...
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Nikonova, Aleksa ...
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Rybalkin, Sergei
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Aldobaev, Vladim ...
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Smirnov, Valeriy
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Shershakova, Nad ...
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Petukhova, Olga
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Kudlay, Dmitriy
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Shatilov, Artem
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Timofeeva, Anast ...
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Campana, Raffael ...
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Udin, Sergei
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Skvortsova, Vero ...
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- MEDICAL AND HEALTH SCIENCES
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Allergy. Europea ...
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Stockholm University
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Karolinska Institutet