Search: onr:"swepub:oai:DiVA.org:su-225804" >
A Method for Using ...
-
Nebogatova, Jekaterina
(author)
A Method for Using Cell-Penetrating Peptides for Loading Plasmid DNA into Secreted Extracellular Vesicles
- Article/chapterEnglish2023
Publisher, publication year, extent ...
Numbers
-
LIBRIS-ID:oai:DiVA.org:su-225804
-
https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-225804URI
-
https://doi.org/10.3390/biom13121751DOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
The low bioavailability and high toxicity of plasmid DNA (pDNA)-based therapeutics pose challenges for their in vivo application. Extracellular vesicles (EVs) have great potential to overcome these limitations, as they are biocompatible native cargo carriers. Various methods for loading pDNA into EVs, including electroporation, sonication, and co-incubation, have been previously investigated, but their success has been questionable. In this study, we report a unique method for loading EVs with pDNA through transient transfection using cell-penetrating peptides (CPPs). With this method, we found a 104-fold increase in the expression levels of the luciferase reporter protein in recipient cells compared to the untreated cells. These data point to the high transfection efficacy and bioavailability of the delivered encapsulated nucleic acid. Furthermore, the in vivo experimental data indicate that the use of pDNA-loaded EVs as native delivery vehicles reduces the toxic effects associated with traditional nucleic acid (NA) delivery and treatment.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Hark, Heleri Heike
(author)
-
Puskar, Anett
(author)
-
Porosk, Ly
(author)
-
Guazzi, Paolo
(author)
-
Dowaidar, Moataz
(author)
-
Langel, ÜloStockholms universitet,Institutionen för biokemi och biofysik,University of Tartu, Estonia(Swepub:su)ulo
(author)
-
Kurrikoff, Kaido
(author)
-
Stockholms universitetInstitutionen för biokemi och biofysik
(creator_code:org_t)
Related titles
-
In:Biomolecules13:122218-273X
Internet link
Find in a library
To the university's database