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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 02809naa a2200433 4500 | |
| 001 | oai:DiVA.org:su-225804 | |
| 003 | SwePub | |
| 008 | 240123s2023 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-2258042 URI |
| 024 | 7 | a https://doi.org/10.3390/biom131217512 DOI |
| 040 | a (SwePub)su | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Nebogatova, Jekaterina4 aut |
| 245 | 1 0 | a A Method for Using Cell-Penetrating Peptides for Loading Plasmid DNA into Secreted Extracellular Vesicles |
| 264 | 1 | c 2023 |
| 338 | a print2 rdacarrier | |
| 520 | a The low bioavailability and high toxicity of plasmid DNA (pDNA)-based therapeutics pose challenges for their in vivo application. Extracellular vesicles (EVs) have great potential to overcome these limitations, as they are biocompatible native cargo carriers. Various methods for loading pDNA into EVs, including electroporation, sonication, and co-incubation, have been previously investigated, but their success has been questionable. In this study, we report a unique method for loading EVs with pDNA through transient transfection using cell-penetrating peptides (CPPs). With this method, we found a 104-fold increase in the expression levels of the luciferase reporter protein in recipient cells compared to the untreated cells. These data point to the high transfection efficacy and bioavailability of the delivered encapsulated nucleic acid. Furthermore, the in vivo experimental data indicate that the use of pDNA-loaded EVs as native delivery vehicles reduces the toxic effects associated with traditional nucleic acid (NA) delivery and treatment. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
| 653 | a extracellular vesicles | |
| 653 | a gene therapy | |
| 653 | a nucleic acid therapeutics | |
| 653 | a drug delivery system | |
| 653 | a cell-penetrating peptides | |
| 700 | 1 | a Hark, Heleri Heike4 aut |
| 700 | 1 | a Puskar, Anett4 aut |
| 700 | 1 | a Porosk, Ly4 aut |
| 700 | 1 | a Guazzi, Paolo4 aut |
| 700 | 1 | a Dowaidar, Moataz4 aut |
| 700 | 1 | a Langel, Ülou Stockholms universitet,Institutionen för biokemi och biofysik,University of Tartu, Estonia4 aut0 (Swepub:su)ulo |
| 700 | 1 | a Kurrikoff, Kaido4 aut |
| 710 | 2 | a Stockholms universitetb Institutionen för biokemi och biofysik4 org |
| 773 | 0 | t Biomoleculesg 13:12q 13:12x 2218-273X |
| 856 | 4 | u https://doi.org/10.3390/biom13121751y Fulltext |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-225804 |
| 856 | 4 8 | u https://doi.org/10.3390/biom13121751 |
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