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Peak purity determi...
Peak purity determination with principal component analysis of high-performance liquid chromatography-diode array detection data
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- Wiberg, Kent (author)
- Stockholms universitet,Institutionen för analytisk kemi
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Andersson, Mattias (author)
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Hagman, Anders (author)
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- Jacobsson, Sven P. (author)
- Stockholms universitet,Institutionen för analytisk kemi
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(creator_code:org_t)
- Elsevier BV, 2004
- 2004
- English.
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In: Journal of Chromatography A. - : Elsevier BV. - 0021-9673 .- 1873-3778. ; 1029:1-2, s. 13-20
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- A method is proposed for the determination of chromatographic peak purity by means of principal component analysis (PCA) of high-performance liquid chromatography with diode array detection (HPLC–DAD) data. The method is exemplified with analysis of binary mixtures of lidocaine and prilocaine with different levels of separation. Lidocaine and prilocaine have very similar spectra and the chromatograms used had substantial peak overlap. The samples analysed contained a constant amount of lidocaine and a minor amount of prilocaine (0.02–2 conc.%) and hence the focus was on determining the purity of the lidocaine peak in the presence of much smaller levels of prilocaine. The peak purity determination was made by examination of relative observation residuals, scores and loadings from the PCA decomposition of DAD data over a chromatographic peak. As a reference method, the functions for peak purity analysis in the chromatographic data system used (Chromeleon) were applied. The PCA method showed good results at the same level as the detection limit of baseline-separated prilocaine, outperforming the methods in Chromeleon by a factor of ten. There is a discussion of the interpretation of the result, with some comparisons with evolving factor analysis (EFA). The main advantage of the PCA method for determination of peak purity over methods like EFA lies in its simplicity, short time of calculation and ease of use.
Keyword
- Principal component analysis
- Peak purity determination
- Relative observation residuals
- Scores
- Loadings
Publication and Content Type
- ref (subject category)
- art (subject category)
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