SwePub
Sök i LIBRIS databas

  Extended search

onr:"swepub:oai:DiVA.org:su-229564"
 

Search: onr:"swepub:oai:DiVA.org:su-229564" > Elucidation of the ...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Elucidation of the O-antigen structure of Escherichia coli O93 and characterization of its biosynthetic genes

Furevi, Axel, 1992- (author)
Stockholms universitet,Institutionen för organisk kemi
Ståhle, Jonas, 1985- (author)
Stockholms universitet,Institutionen för organisk kemi
Muheim, Claudio, 1987- (author)
Stockholms universitet,Institutionen för biokemi och biofysik
show more...
Gkotzis, Spyridon (author)
Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
Daley, Daniel (author)
Stockholms universitet,Institutionen för biokemi och biofysik
Udekwu, Klas (author)
Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,Swedish University of Agriculture, Sweden
Widmalm, Göran, 1958- (author)
Stockholms universitet,Institutionen för organisk kemi
show less...
 (creator_code:org_t)
2022-10-14
2023
English.
In: Glycobiology. - : Oxford University Press (OUP). - 0959-6658 .- 1460-2423. ; 33:4, s. 289-300
  • Journal article (peer-reviewed)
Abstract Subject headings
Close  
  • The structure of the O-antigen from the international reference strain Escherichia coli O93:-:H16 has been determined. A nonrandom modal chain-length distribution was observed for the lipopolysaccharide, a pattern which is typical when long O-specific polysaccharides are expressed. By a combination of (i) bioinformatics information on the gene cluster related to O-antigen synthesis including putative function on glycosyl transferases, (ii) the magnitude of NMR coupling constants of anomeric protons, and (iii) unassigned 2D H-1, C-13-HSQC, and H-1,H-1-TOCSY NMR spectra it was possible to efficiently elucidate the structure of the carbohydrate polymer in an automated fashion using the computer program CASPER. The polysaccharide also carries O-acetyl groups and their locations were determined by 2D NMR experiments showing that similar to 1/2 of the population was 2,6-di-O-acetylated, similar to 1/4 was 2-O-acetylated, whereas similar to 1/4 did not carry O-acetyl group(s) in the 3-O-substituted mannosyl residue of the repeating unit. The structure of the tetrasaccharide repeating unit of the O-antigen is given by: -> 2)-beta-D-Manp-(1 -> 3)-beta-D-Manp2Ac6Ac-(1 -> 4)-beta-D-GlcpA-(1 -> 3)-alpha-D-GlcpNAc-(1 ->, which should also be the biological repeating unit and it shares structural elements with capsular polysaccharides from E. coli K84 and K50. The structure of the acidic O-specific polysaccharide from Cellulophaga baltica strain NN015840(T) differs to that of the O-antigen from E. coli O93 by lacking the O-acetyl group at O6 of the O-acetylated mannosyl residue.

Subject headings

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Keyword

bioinformatics
CarbBuilder
CASPER
lipopolysaccharide
NMR spectroscopy

Publication and Content Type

ref (subject category)
art (subject category)

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Search outside SwePub

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view