Search: onr:"swepub:oai:DiVA.org:su-24380" >
New Escherichia col...
New Escherichia coli outer membrane proteins identified through prediction and experimental verification
-
- Marani, Paula (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,University of Bologna, Italy
-
- Wagner, Samuel (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
-
- Baars, Louise (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
-
show more...
-
Genevaux, Pierre (author)
-
- de Gier, Jan-Willem (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
-
- Nilsson, IngMarie (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
-
Casadio, Rita (author)
-
- von Heijne, Gunnar (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
-
show less...
-
(creator_code:org_t)
- 2006-03-07
- 2006
- English.
-
In: Protein Science. - : Wiley. - 0961-8368 .- 1469-896X. ; 15:4, s. 884-889
- Related links:
-
https://europepmc.or...
-
show more...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Many new Escherichia coli outer membrane proteins have recently been identified by proteomics techniques. However, poorly expressed proteins and proteins expressed only under certain conditions may escape detection when wild-type cells are grown under standard conditions. Here, we have taken a complementary approach where candidate outer membrane proteins have been identified by bioinformatics prediction, cloned and overexpressed, and finally localized by cell fractionation experiments. Out of eight predicted outer membrane proteins, we have confirmed the outer membrane localization for five-YftM, YaiO, YfaZ, CsgF, and YliI - and also provide preliminary data indicating that a sixth -YfaL- may be an outer membrane autotransporter.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- outer membrane protein
- bioinformatics
- SecB
- autotransporter
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database