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Relevance of the N-...
Relevance of the N-terminal NLS-like sequence for membrane interactions of the Prion protein
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- Oglęcka, Kamila (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Lundberg, Pontus (author)
- Stockholms universitet,Institutionen för neurokemi
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- Magzoub, Mazin (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Eriksson, L. E. Göran (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Langel, Ülo (author)
- Stockholms universitet,Institutionen för neurokemi
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- Gräslund, Astrid (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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(creator_code:org_t)
- Elsevier BV, 2007
- 2007
- English.
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In: Biochimica et Biophysica Acta. MR. Reviews on Biomembranes. - : Elsevier BV. - 0304-4157 .- 1879-257X. ; 1778:1, s. 206-213
- Related links:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- We investigated the nuclear localization-like sequence KKRPKP, corresponding to the residues 23–28 in the mouse prion protein (mPrP), for its membrane perturbation activity, by comparing effects of two mPrP-derived peptides, corresponding to residues 1–28 (mPrPp(1–28)) and 23–50 (mPrPp(23–50)), respectively. In erythrocytes, mPrPp(1–28) induced 60% haemoglobin leakage after 30 min, whereas mPrPp(23–50) had negligible effects. In calcein-entrapping, large unilamellar vesicles (LUVs), similar results were obtained. Cytotoxicity estimated by lactate dehydrogenase leakage from HeLa cells, was found to be 12% for 50 μM mPrPp(1–28), and 1% for 50 μM mPrPp(23–50). Circular dichroism spectra showed structure induction of mPrPp(1–28) in the presence of POPC:POPG (4:1) and POPC LUVs, while mPrPp(23–50) remained a random coil. Membrane translocation studies on live HeLa cells showed mPrPp(1–28) co-localizing with dextran, suggesting fluid-phase endocytosis, whereas mPrPp(23–50) hardly translocated at all. We conclude that the KKRPKP-sequence is not sufficient to cause membrane perturbation or translocation but needs a hydrophobic counterpart.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Other Basic Medicine (hsv//eng)
Keyword
- Prion protein
- Membrane translocation
- Calcein leakage
- Endosomal escape
- NLS-like sequence
- Haemoglobin leakage
- Chemistry
- Kemi
Publication and Content Type
- ref (subject category)
- art (subject category)
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